NQO1 Antibody

NQO1 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1E5G7

Cat no : 67240-1-Ig

Synonyms

Azoreductase, DHQU, DIA4, DT diaphorase, DTD, Menadione reductase, NQO1, Phylloquinone reductase, QR1, Quinone reductase 1



Tested Applications

Positive WB detected inHepG2 cells, HSC-T6 cells, HeLa cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite®488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

67240-1-Ig targets NQO1 in WB, IHC, ELISA applications and shows reactivity with Human, Rat samples.

Tested Reactivity Human, Rat
Cited Reactivity mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen NQO1 fusion protein Ag28933
Full Name NAD(P)H dehydrogenase, quinone 1
Calculated molecular weight 274aa,31 kDa
Observed molecular weight 29-31 kDa
GenBank accession number BC007659
Gene symbol NQO1
Gene ID (NCBI) 1728
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

NQO1, also named as DIA4, NMOR1, DTD and QR1, belongs to the NAD(P)H dehydrogenase (quinone) family. This enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. It is known to be involved in benzene metabolism. In human studies of ozone exposure, polymorphisms in oxidative stress genes (NQO1, GSTM1, GSTP1) modify respiratory symptoms, lung function, biomarkers and risk of asthma. (PMID:18511640; 18848868 )

Protocols

Product Specific Protocols
WB protocol for NQO1 antibody 67240-1-IgDownload protocol
IHC protocol for NQO1 antibody 67240-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Biomed Pharmacother

Induction of Nrf2 pathway by Dendrobium nobile Lindl. alkaloids protects against carbon tetrachloride induced acute liver injury.

Authors - Shiyue Li