Tested Applications
| Positive WB detected in | mouse skeletal muscle tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84458-4-RR targets P2RX6 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag12331 Product name: Recombinant human P2RX6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-330 aa of BC033488 Sequence: MGSPGATTGWGLLDYKTEKYVMTRNWRVGALQRLLQFGIVVYVVGWALLAKKGYQERDLEPQFSIITKLKGVSVTQIKELGNRLWDVADFVKPPQGENVFFLVTNFLVTPAQVQGRCPEHPSVPLANCWVDEDCPEGEGGTHSHGVKTGQCVVFNGTHRTCEIWSWCPVESGVVPSRPLLAQAQNFTLFIKNTVTFSKFNFSKSNALETWDPTYFKHCRYEPQFSPYCPVFRIGDLVAKAGGTFEDLALLGGSVGIRVHWDCDLDTGDSGCWPHYSFQLQEKSYNFRTATHWWEQPGVEARTLLKLYGIRFDILVTGQAGKFGLIPTAVT Predict reactive species |
| Full Name | purinergic receptor P2X, ligand-gated ion channel, 6 |
| Calculated Molecular Weight | 431 aa, 48 kDa |
| Observed Molecular Weight | 45 kDa |
| GenBank Accession Number | BC033488 |
| Gene Symbol | P2RX6 |
| Gene ID (NCBI) | 9127 |
| RRID | AB_3671983 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | O15547 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The P2 receptors have two subfamilies in mammalian cells, P2X and P2Y. P2X family of ligand-gated ion channel receptors have seven members, P2RX1-7, which all constitute non-selective cation channels proposed to be functional under a homo- or heterotrimeric association. P2RX6 is predominantly expressed in skeletal muscle, and regulated by p53. It's reported that ATP might increase renal cell carcinoma cells migration and invasion through P2RX6.(PMID: 31159832, PMID:9242461)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for P2RX6 antibody 84458-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
