PCK1 Monoclonal antibody

PCK1 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

mouse, rat, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2F11B5

Cat no : 66862-1-Ig

Synonyms

PCK1, PEPCK C, PEPCK1, PEPCKC



Tested Applications

Positive WB detected inpig kidney tissue, rat liver tissue, pig liver tissue
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

66862-1-Ig targets PCK1 in WB, IHC, ELISA applications and shows reactivity with mouse, rat, pig samples.

Tested Reactivity mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PCK1 fusion protein Ag9720
Full Name phosphoenolpyruvate carboxykinase 1 (soluble)
Calculated molecular weight 622 aa, 69 kDa
Observed molecular weight 65-69 kDa
GenBank accession numberBC023978
Gene symbol PCK1
Gene ID (NCBI) 5105
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PCK1(Phosphoenolpyruvate carboxykinase, cytosolic) is also named as PEPCK1 and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. It is also a main control point for the regulation of gluconeogenesis. In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one. Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (C-PEPCKD).

Protocols

Product Specific Protocols
WB protocol for PCK1 antibody 66862-1-IgDownload protocol
IHC protocol for PCK1 antibody 66862-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols