PER2 Polyclonal antibody

PER2 Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 20359-1-AP

Synonyms

FASPS, hPER2, KIAA0347, PER2, period homolog 2 (Drosophila)



Tested Applications

Positive WB detected inBxPC-3 cells, Y79 cells, K-562 cells, L02 cells
Positive IP detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Sample-dependent, check data in validation data gallery

Product Information

20359-1-AP targets PER2 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity goat, human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name period homolog 2 (Drosophila)
Calculated molecular weight 137 kDa
Observed molecular weight 136 kDa
GenBank accession numberNM_022817
Gene symbol PER2
Gene ID (NCBI) 8864
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PER2, also named as KIAA0347, is a component of the circadian clock mechanism which is essential for generating circadian rhythms. PER2 is a negative element in the circadian transcriptional loop. PER2 influences clock function by interacting with other circadian regulatory proteins and transporting them to the nucleus. It negatively regulates CLOCK|NPAS2-BMAL1|BMAL2-induced transactivation. Defects in PER2 are a cause of familial advanced sleep-phase syndrome (FASPS) which is characterized by very early sleep onset and offset. The antibody is specific to PER2. It can recognize isoform1 and isoform2 of PER2.

Protocols

Product Specific Protocols
WB protocol for PER2 antibody 20359-1-APDownload protocol
IP protocol for PER2 antibody 20359-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Hum Cell

Pathophysiological significance of clock genes BMAL1 and PER2 as erythropoietin-controlling factors in acute blood hemorrhage.

Authors - Naoto Tani
humanWB

Cell Death Dis

The circadian clock gene Bmal1 facilitates cisplatin-induced renal injury and hepatization.

Authors - Min Zha
humanWB

Cell Death Dis

hPER3 promotes adipogenesis via hHSP90AA1-mediated inhibition of Notch1 pathway.

Authors - Xinxing Wan
humanIP, IF

Int J Mol Sci

Phosphorylation of GAPVD1 Is Regulated by the PER Complex and Linked to GAPVD1 Degradation.

Authors - Hussam Ibrahim
WB

Oncol Lett

Construction of recombinant pEGFP-N1-hPer2 plasmid and its expression in osteosarcoma cells.

Authors - Anyuan Cheng
goatWB, IF

J Dairy Sci

Ruminal epithelial cell proliferation and short-chain fatty acid transporters in vitro are associated with abundance of period circadian regulator 2 (PER2).

Authors - Jian Gao
  • KD Validated