PEX14 Polyclonal antibody

PEX14 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IP, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 10594-1-AP

Synonyms

NAPP2, Peroxin 14, PEX14, Pex14p, PTS1 receptor docking protein



Tested Applications

Positive WB detected inA431 cells, HEK-293T cells, HeLa cells, SKOV-3 cells
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, C2C12 cells, COS7 cells, HeLa cells, NIH/3T3 cells
Positive FC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10594-1-AP targets PEX14 in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Reactivity human, mouse, rat, monkey
Cited Reactivity human, monkey, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PEX14 fusion protein Ag0932
Full Name peroxisomal biogenesis factor 14
Calculated molecular weight 41 kDa
Observed molecular weight 57 kDa
GenBank accession numberBC006327
Gene symbol PEX14
Gene ID (NCBI) 5195
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PEX14 (peroxisomal biogenesis factor 14) is a peroxisomal membrane protein that is essential for protein docking onto the peroxisomes. It is a central component of the peroxisimal matrix protein import machinery and interacts with PEX5 and PEX19. PEX14 is ubiquitously expressed and defects in PEX14 are the cause of peroxisome biogenesis disorder complementation group K (PBD-CGK). This antibody can be used to detect endogenous PEX14 with an apparent molecular weight of 57 kDa (PMID: 16449325; 9653144) and recognize peroxisomal structures in human, monkey and mouse cells.

Protocols

Product Specific Protocols
WB protocol for PEX14 antibody 10594-1-APDownload protocol
IHC protocol for PEX14 antibody 10594-1-APDownload protocol
IF protocol for PEX14 antibody 10594-1-APDownload protocol
IP protocol for PEX14 antibody 10594-1-APDownload protocol
FC protocol for PEX14 antibody 10594-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Biochim Biophys Acta

Peroxisome deficiency-induced ER stress and SREBP-2 pathway activation in the liver of newborn PEX2 knock-out mice.

Authors - Kovacs Werner J WJ
humanIF

JIMD Rep

Lymphoblastoid cell lines for diagnosis of peroxisome biogenesis disorders.

Authors - Grønborg Sabine S
humanIF

Mol Biol Cell

Novel regulatory roles of Mff and Drp1 in E3 ubiquitin ligase MARCH5-dependent degradation of MiD49 and Mcl1 and control of mitochondrial dynamics.

Authors - Edward Cherok
mouseWB

Methods Mol Biol

Isolation of Peroxisomes from Mouse Brain Using a Continuous Nycodenz Gradient: A Comparison to the Isolation of Liver and Kidney Peroxisomes.

Authors - Miriam J Schönenberger
IF

Cell Death Dis

A cross-talk between epithelium and endothelium mediates human alveolar-capillary injury during SARS-CoV-2 infection.

Authors - Peng Wang
humanIF

Am J Med Genet A

Typical cMRI pattern as diagnostic clue for D-bifunctional protein deficiency without apparent biochemical abnormalities in plasma.

Authors - Gr?nborg Sabine S

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tim (Verified Customer) (04-09-2021)

20ug cell lysate loaded and separated by SDS-PAGE prior to transfer. Antibody incubation overnight in cold room.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/2000
  • Cell Tissue Type: HEK293
PEX14 Antibody Western Blot validation (1/2000 dilution) in HEK293 (Cat no:10594-1-AP)
FH

Tom (Verified Customer) (03-30-2021)

HEK293 cells fixed in methanol at -20 degrees. PEX14 antibody visualized using anti-rabbit 594 (red) secondary antibody.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: HEK293
PEX14 Antibody Immunofluorescence validation (1:100 dilution) in HEK293 (Cat no:10594-1-AP)
FH

Chun (Verified Customer) (12-05-2019)

A good antibody

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: HeLa cells