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  • KD/KO Validated

PRDX4 Antibody

PRDX4 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 10703-1-AP

Synonyms

Antioxidant enzyme AOE372, AOE37 2, peroxiredoxin 4, Peroxiredoxin IV, PRDX4, PRX 4, Prx IV, Thioredoxin peroxidase AO372



Tested Applications

Positive WB detected inHepG2 cells, Jurkat
Positive IP detected inHepG2 cells
Positive IHC detected inhuman pancreas cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10703-1-AP targets PRDX4 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Reactivity human, mouse, rat
Cited Species human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRDX4 fusion protein Ag1177
Full Name peroxiredoxin 4
Calculated molecular weight 31 kDa
Observed molecular weight 30 kDa
GenBank accession number BC007107
Gene symbol PRDX4
Gene ID (NCBI) 10549
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PRDX4 (Peroxiredoxin-4) is also named as AOE37-2, Prx-IV and belongs to the AhpC/TSA family. PRDX4 is associated with acrosome formation during rat spermatogenesis and has a protective role in the male reproductive tract, because the phenotype of mice lacking this isoform includes testicular atrophy and increased sperm DNA damage (PMID:20864641). It is initially synthesized as a membrane-binding 31-kDa protein and processed into a 27-kDa secretory form and is discarded with the residual bodies (PMID:19208552). PRDX4 is a pentamer of dimers (PMID:23025503). This antibody is specific to PRDX4.

Protocols

Product Specific Protocols
WB protocol for PRDX4 antibody 10703-1-APDownload protocol
IHC protocol for PRDX4 antibody 10703-1-APDownload protocol
IF protocol for PRDX4 antibody 10703-1-APDownload protocol
IP protocol for PRDX4 antibody 10703-1-APDownload protocol
FC protocol for PRDX4 antibody 10703-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Br J Pharmacol

Luteolin ameliorates rat myocardial ischemia-reperfusion injury through peroxiredoxin II activation.

Authors - Bo Wei
humanWB,IHC

Hepatobiliary Pancreat Dis Int

Comparative proteomic analysis of differentially expressed proteins in human pancreatic cancer tissue.

Authors - Chen Jian-Hua JH
ratWB

Can J Physiol Pharmacol

Galectin-3 inhibition attenuates doxorubicin-induced cardiac dysfunction by upregulating the expression of peroxiredoxin-4.

Authors - Yunpeng Tian
  • KD Validated
humanWB

Proteomics Clin Appl

Quantitative proteomic analysis of a paired human liver healthy versus carcinoma cell lines with the same genetic background to identify potential hepatocellular carcinoma markers.

Authors - Bao Huimin H
humanWB,IF

J Cell Biochem

Nucleophosmin regulates intracellular oxidative stress homeostasis via antioxidant PRDX6.

Authors - Guo-Yan Liu
mouseWB

Biomed Pharmacother

Intrabody against prolyl hydroxylase 2 ameliorates acetaminophen-induced acute liver injury in mice via concomitant promotion of angiogenesis and redox homeostasis.

Authors - Liangzhong Zhao