PRICKLE1 Antibody

PRICKLE1 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 22589-1-AP

Synonyms

EPM1B, prickle homolog 1 (Drosophila), Prickle like protein 1, PRICKLE1, RILP



Tested Applications

Positive WB detected inmouse brain tissue, Neuro-2a cells
Positive IP detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

The 22589-1-AP immunogen is Fusion Protein PRICKLE1 protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRICKLE1 fusion protein Ag18407
Full Name prickle homolog 1 (Drosophila)
Calculated molecular weight 831aa,94 kDa
Observed molecular weight 94 kDa, 55-60 kDa
GenBank accession number BC114939
Gene symbol PRICKLE1
Gene ID (NCBI) 144165
Conjugate Unconjugated
Form Liquid
Purification Method Antigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PRICKLE1 antibody 22589-1-APDownload protocol
IP protocol for PRICKLE1 antibody 22589-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
chickenWB

Development

Coupling of apical-basal polarity and PCP to interpret the Wnt signaling gradient and orient feather branch.

Authors - Jianqiong Lin

Glia

The formation of paranodal spirals at the ends of CNS myelin sheaths requires the planar polarity protein Vangl2.

Authors - Andrew A Jarjour