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  • Western Blot (WB) analysis of various lysates using PSMB8 Recombinant antibody (86241-1-RR)

    WB analysis using 86241-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86241-1-RR (PSMB8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PSMB8 Recombinant antibody (86241-1-RR)

    IF Staining of HeLa using 86241-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSMB8 antibody (86241-1-RR, Clone: 250865C7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 86241-1-RR

    Affinity and Kinetic Characterization of 86241-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86241-1-RR against Human PSMB8 were performed. The affinity constant is below 1 pM.

PSMB8 Recombinant monoclonal antibody

PSMB8 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86241-1-RR
Clone No.250865C7

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

EC:3.4.25.1, LMP7, Proteasome subunit beta type 8, Proteasome subunit beta type-8, Proteasome subunit beta-5i

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inJurkat cells, HuT 78 cells, MOLT-4 cells, ACHN cells, Raji cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86241-1-RR targets PSMB8 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag6660

Product name: Recombinant human PSMB8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-272 aa of BC001114

Sequence: MLIGTPTPRDTTPSSWLTSSLLVEAAPLDDTTLPTPVSSGCPGLEPTEFFQSLGGDGERNVQIEMAHGTTTLAFKFQHGVIAAVDSRASAGSYISALRVNKVIEINPYLLGTMSGCAADCQYWERLLAKECRLYYLRNGERISVSAASKLLSNMMCQYRGMGLSMGSMICGWDKKGPGLYYVDEHGTRLSGNMFSTGSGNTYAYGVMDSGYRPNLSPEEAYDLGRRAIAYATHRDSYSGGVVNMYHMKEDGWVKVESTDVSDLLHQYREANQ

Predict reactive species
Full Name proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctional peptidase 7)
Calculated Molecular Weight 30 kDa
Observed Molecular Weight 23 kDa, 30 kDa
GenBank Accession NumberBC001114
Gene Symbol PSMB8
Gene ID (NCBI) 5696
Conjugate Unconjugated
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP28062
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for PSMB8 antibody 86241-1-RRDownload protocol
WB protocol for PSMB8 antibody 86241-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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