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RARS Monoclonal antibody

RARS Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1C8G4

Cat no : 66815-1-Ig

Synonyms

Arginine tRNA ligase, arginyl tRNA synthetase, ArgRS, DALRD1, RARS

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RARS Monoclonal antibody (66815-1-Ig)

    WB analysis using 66815-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66815-1-Ig (RARS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using RARS Monoclonal antibody (66815-1-Ig)

    WB analysis using 66815-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66815-1-Ig (RARS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RARS Monoclonal antibody (66815-1-Ig)

    WB analysis of HeLa using 66815-1-Ig

    WB result of RARS antibody (66815-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RARS transfected HeLa cells.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RARS Monoclonal antibody (66815-1-Ig)

    IF Staining of HeLa using 66815-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RARS antibody (66815-1-Ig, Clone: 1C8G4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, HSC-T6 cells, MCF-7 cells, A431 cells, HEK-293 cells, HepG2 cells, NIH/3T3 cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:3000-1:8000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66815-1-Ig targets RARS in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen RARS fusion protein Ag26399
Full Name arginyl-tRNA synthetase
Calculated Molecular Weight 75 kDa
Observed Molecular Weight 67 kDa
GenBank Accession NumberBC000528
Gene Symbol RARS
Gene ID (NCBI) 5917
RRIDAB_2882158
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RARS, also named ArgRS (arginyl-tRNA synthetase), belongs to the class-I aminoacyl-tRNA synthetase family which catalyze the aminoacylation of tRNA by their cognate amino acid. In higher eukaryotes, one of the two arginyl-tRNA synthetases (ArgRSs) has evolved to have an extended N-terminal domain that plays a crucial role in protein synthesis and cell growth and in integration into the multisynthetase complex (MSC) (PMID:25288775). RARS has two isoforms with the molecular weight of 75 and 67 kDa.

Protocols

Product Specific Protocols
WB protocol for RARS antibody 66815-1-IgDownload protocol
IF protocol for RARS antibody 66815-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols