Validation Data Gallery
|Positive WB detected in
|A431 cells, HEK-293 cells, A549 cells, K-562 cells, PC-3 cells
|Positive IP detected in
|Positive IF detected in
|Positive FC detected in
|Western Blot (WB)
|WB : 1:2000-1:10000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IF : 1:50-1:500
|Flow Cytometry (FC)
|FC : 0.20 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
10144-2-AP targets STAT1 in WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with human samples.
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|STAT1 fusion protein Ag0199
|signal transducer and activator of transcription 1, 91kDa
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
What is the molecular weight of STAT1? Are there any isoforms of STAT1? Is STAT1 post-translationally modified?
The molecular weight of STAT1 is 91 kDa for full-length STAT1α and 84 kDa for STAT1β, which is devoid of the C-terminal activation domain (PMID: 1502203). Phosphorylation of STAT1 regulates STAT1 activity. Phosphorylation of Tyr701 by Janus kinases (JAKs) triggers STAT1 homo- and hetero-dimerization with other STAT family members and also causes nuclear translocation, while phosphorylation of Ser727 is needed for full activation (PMID: 10851062). STAT1β lacks Ser727 and has therefore been considered to be inactive or acting in a dominant negative manner to STAT1α, although it has been further shown to be capable of transcription activation in vivo (PMID: 24710278).
What is the subcellular localization of STAT1?
STAT1 is present in the cytoplasm and in the nucleus. Translocation of STAT1 from the cytoplasm to the nucleus is required for its regulation of gene expression and is dependent on phosphorylation (see above). However, low levels of unphosphorylated STAT1, often referred to as U-STAT1, can also be found in the nucleus, where U-STAT1 regulates the stability of heterochromatin by binding to chromatin in monomeric or dimeric states (PMID: 18840523).
What is the tissue expression pattern of STAT1?
STAT1 is ubiquitously expressed.
|Product Specific Protocols
|WB protocol for STAT1 antibody 10144-2-AP
|IHC protocol for STAT1 antibody 10144-2-AP
|IF protocol for STAT1 antibody 10144-2-AP
|IP protocol for STAT1 antibody 10144-2-AP
|Click here to view our Standard Protocols
Opioid-induced fragile-like regulatory T cells contribute to withdrawal
ID1 expressing macrophages support cancer cell stemness and limit CD8+ T cell infiltration in colorectal cancer
Sci Transl Med
TRIB3 reduces CD8+ T cell infiltration and induces immune evasion by repressing the STAT1-CXCL10 axis in colorectal cancer.
Cell-Free Extracts from Human Fat Tissue with a Hyaluronan-Based Hydrogel Attenuate Inflammation in a Spinal Cord Injury Model through M2 Microglia/Microphage Polarization.
Osteoblasts secrete Cxcl9 to regulate angiogenesis in bone.
Inhibition of STAT3-ferroptosis negative regulatory axis suppresses tumor growth and alleviates chemoresistance in gastric cancer.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Paul (Verified Customer) (11-24-2022)
Clear band at the expected size range
Alejandro (Verified Customer) (08-22-2022)
Works fine for both WB, with clear bands, and IF, which shows a high specific staining
Slavica (Verified Customer) (10-04-2021)
The antibody worked very well!!!