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STAT3 Monoclonal antibody

STAT3 Monoclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3G2D12

Cat no : 60199-1-Ig

Synonyms

Acute phase response factor, APRF, HIES, STAT3



Tested Applications

Positive WB detected inHeLa cells, HeLa
Positive IP detected inHeLa cells
Positive IHC detected inhuman cervical cancer tissue, human heart tissue, human breast cancer tissue, human endometrial cancer tissue, human liver tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 60199-1-Ig is STAT3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen STAT3 fusion protein Ag0360
Full Name signal transducer and activator of transcription 3 (acute-phase response factor)
Calculated molecular weight 770 aa, 88 kDa
Observed molecular weight 88 kDa
GenBank accession numberBC000627
Gene symbol STAT3
Gene ID (NCBI) 6774
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3. This antibody is a mouse monoclonal antibody raised against residues near the N terminus of human STAT3. STAT3 exists three isoforms and the molecular weight of each isoform respectively is 83 kDa, 87 kDa and 88 kDa.

Protocols

Product Specific Protocols
WB protocol for STAT3 antibody 60199-1-IgDownload protocol
IHC protocol for STAT3 antibody 60199-1-IgDownload protocol
IF protocol for STAT3 antibody 60199-1-IgDownload protocol
IP protocol for STAT3 antibody 60199-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Brain Res Bull

Inhibition of Connexin43 Hemichannels with Gap19 Protect-s Cerebral Ischemia/Reperfusion Injury via the JAK2/STAT3 Pathway in Mice.

Authors - Beilei Chen
humanWB

Mol Carcinog

Exosome-transmitted p120-catenin suppresses hepatocellular carcinoma progression via STAT3 pathways.

Authors - Zhangjun Cheng
humanWB,IHC

Cell Death Dis

CCL5 derived from tumor-associated macrophages promotes prostate cancer stem cells and metastasis via activating β-catenin/STAT3 signaling.

Authors - Renlun Huang
humanWB

Mol Med Rep

Effect of modified Xiaochaihu decoction‑containing serum on HepG2.2.15 cells via the JAK2/STAT3 signaling pathway.

Authors - Shaofang Chen
ratWB,IHC

BMC Cancer

Postoperative resveratrol administration improves prognosis of rat orthotopic glioblastomas.

Authors - Xue Song
humanWB

Front Pharmacol

Berberine Maintains the Neutrophil N1 Phenotype to Reverse Cancer Cell Resistance to Doxorubicin.

Authors - Shuhui Zhang