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  • KD/KO Validated

SYNJ2BP Antibody

SYNJ2BP Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat no : 15666-1-AP

Synonyms

ARIP2, OMP25, synaptojanin 2 binding protein, SYNJ2BP



Tested Applications

Positive WB detected inA549 cells, mouse kidney tissue, mouse lung tissue, human heart tissue
Positive IP detected inmouse lung tissue
Positive IHC detected inhuman stomach cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

15666-1-AP targets SYNJ2BP in IF, IHC, IP, WB, ELISA applications and shows reactivity with human, mouse samples.

Reactivity human, mouse
Cited Species human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SYNJ2BP fusion protein Ag8130
Full Name synaptojanin 2 binding protein
Calculated molecular weight 145aa,16 kDa
Observed molecular weight 16 kDa
GenBank accession number BC007704
Gene symbol SYNJ2BP
Gene ID (NCBI) 55333
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

Activin, a member of the TGF-beta superfamily, plays important roles in reproductive tissues as a stimulator of follicle-stimulating hormone (FSH) secretion and inhibits the proliferation of breast cancer cells. Activin receptor-interacting protein 2 (ARIP2), also known as synaptojanin-2-binding protein (SYNJ2BP), is located in the outer membrane of mitochondria. ARIP2 is widely distributed in various human tissues, and has been recently identified in mouse tissues as a regulatory protein of activin signal transduction by interacting with activin type II receptors. ARIP2 may be a putative growth-promoting factor involved in breast tumorigenesis and tumor development. A double band of size 15.9 kDa can be detected in Western blot (PMID: 23284606).

Protocols

Product Specific Protocols
WB protocol for SYNJ2BP antibody 15666-1-APDownload protocol
IHC protocol for SYNJ2BP antibody 15666-1-APDownload protocol
IF protocol for SYNJ2BP antibody 15666-1-APDownload protocol
IP protocol for SYNJ2BP antibody 15666-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

J Exp Clin Cancer Res

SYNJ2BP inhibits tumor growth and metastasis by activating DLL4 pathway in hepatocellular carcinoma.

Authors - Xiao Liu
human,mouseWB,IHC,IP

Oncotarget

SYNJ2BP promotes the degradation of PTEN through the lysosome-pathway and enhances breast tumor metastasis via PI3K/AKT/SNAI1 signaling.

Authors - Miao Wang
  • KD Validated
humanWB

Cell Rep

Sam50 Regulates PINK1-Parkin-Mediated Mitophagy by Controlling PINK1 Stability and Mitochondrial Morphology.

Authors - Fenglei Jian