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transgelin/SM22 Polyclonal antibody

transgelin/SM22 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 10493-1-AP

Synonyms

SM22, SM22 alpha, SM22a, SM22alpha, TAGLN, TAGLN1, transgelin



Tested Applications

Positive WB detected inmouse colon tissue, Caco-2 cells, human colon tissue
Positive IHC detected inhuman heart tissue, mouse heart tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10493-1-AP targets transgelin/SM22 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen transgelin/SM22 fusion protein Ag0764
Full Name transgelin
Calculated molecular weight 22 kDa
Observed molecular weight 19-22 kDa
GenBank accession numberBC004927
Gene symbol TAGLN
Gene ID (NCBI) 6876
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The transgelin family is a group of proteins that belong to 22kd actin-related calponin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. This antibody was generated against full length transgelin 1 protein. It can cross-react with other two transgelins based on the sequence similarity.

Protocols

Product Specific Protocols
WB protocol for transgelin/SM22 antibody 10493-1-APDownload protocol
IHC protocol for transgelin/SM22 antibody 10493-1-APDownload protocol
IF protocol for transgelin/SM22 antibody 10493-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Antioxid Redox Signal

Peroxisome proliferator-activated receptor-γ coactivator-1α inhibits vascular calcification through sirtuin 3‒mediated reduction of mitochondrial oxidative stress.

Authors - Han Feng
ratWB

Eur J Pharmacol

Death-associated protein kinase 3 deficiency alleviates vascular calcification via AMPK-mediated inhibition of endoplasmic reticulum stress.

Authors - Ke-Xue Li
humanWB

Nat Commun

Poly(ADP-ribose) polymerase 1 accelerates vascular calcification by upregulating Runx2.

Authors - Cheng Wang
mouseWB,IF

FASEB J

Transactivation domain of Krüppel-like factor 15 negatively regulates angiotensin II-induced adventitial inflammation and fibrosis.

Authors - Yuan-Yuan Lu
ratWB

FASEB J

CTRP13 attenuates vascular calcification by regulating Runx2.

Authors - Yongxia Li
humanWB

Biochem Biophys Res Commun

PMID: 30409428

Authors - Hao Han

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Ying (Verified Customer) (08-19-2019)

very good antibody

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1000
  • Cell Tissue Type: primary mouse cardiomyocytes