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TDP-43 (human specific) Antibody 43 Publications

Mouse Monoclonal| Catalog number: 60019-2-Ig |CloneNo.: 6H6E12

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Con: 160 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, FC, CoIP, ELISA
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Images See all

TDP-43 (human specific) Antibody IHC human brain(FTLD-U) tissue 60019-2-Ig

40X of FTLD-U case stained by 10782-2-AP and 60019-2-Ig, showing dystrophic neurites. (Figs were provided by Linda K. Kwong)

TDP-43 (human specific) Antibody WB MCF-7 cells 60019-2-Ig

Various cells were subjected to SDS PAGE followed by western blot with 60019-2-Ig (TDP-43 (human specific) antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours

TDP-43 (human specific) Antibody IP K-562 cells 60019-2-Ig

IP Result of anti-TDP-43 (human specific) (IP:60019-2-Ig, 5ug; Detection:60019-2-Ig 1:1000) with K-562 cells lysate 1720ug.

TDP-43 (human specific) Antibody FC MCF-7 cells 60019-2-Ig

1X10^6 MCF-7 cells were stained with 0.2ug TDP-43 (human specific) antibody (60019-2-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1500.

Species specificity:
human
Positive WB detected in:
MCF-7 cells
Positive IP detected in:
K-562 cells
Positive IHC detected in:
human brain(FTLD-U) tissue, human pancreas cancer tissue, human gliomas tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected in:
MCF-7 cells
Recommended dilution:
WB : 1:5000-1:100000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:50-1:50000

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Mouse
Purification method:
Caprylic acid/ammonium sulfate precipitation
Isotype:
IgG1
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
fusion protein
Full name:
TAR DNA binding protein
Calculated molecular weight:
43 kDa
Observed molecular weight:
43 kDa
GenBank accession number:
BC001487
Gene ID (NCBI):
23435
Gene symbol
TARDBP
Synonyms
ALS10, TAR DNA binding protein, TAR DNA binding protein 43, TARDBP, TDP 43, TDP43

Background

Transactivation response (TAR) DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43) was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659,19823856, 21666678, 22193176). 60019-2-Ig is a mouse monoclonal antibody recognizing the cleavage product of 20-30 kDa in addition to the native and phosphorylated forms of TDP-43. Immunohistochemical analyses of TDP-43 using this antibody detect both normal diffuse nuclear staining and insoluble inclusions in pathologic tissues. Notably this antibody only recognizes human TDP-43 but not reacts with mouse or rat TDP-43.


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