TDP-43 (human specific) Monoclonal antibody

TDP-43 (human specific) Monoclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IP, IHC, FC, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

6H6E12

Cat no : 60019-2-Ig

Synonyms

ALS10, TAR DNA binding protein, TAR DNA binding protein 43, TARDBP, TDP 43, TDP43



Tested Applications

Positive WB detected inMCF-7 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman brain(FTLD-U) tissue, human pancreas cancer tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:100000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:50000
Sample-dependent, check data in validation data gallery

Product Information

60019-2-Ig targets TDP-43 (human specific) in WB, IP, IHC, FC, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen fusion protein
Full Name TAR DNA binding protein
Calculated molecular weight 43 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC001487
Gene symbol TARDBP
Gene ID (NCBI) 23435
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Transactivation response (TAR) DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43) was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659,19823856, 21666678, 22193176). 60019-2-Ig is a mouse monoclonal antibody recognizing the cleavage product of 20-30 kDa in addition to the native and phosphorylated forms of TDP-43. Immunohistochemical analyses of TDP-43 using this antibody detect both normal diffuse nuclear staining and insoluble inclusions in pathologic tissues. Notably this antibody only recognizes human TDP-43 but not reacts with mouse or rat TDP-43.

Protocols

Product Specific Protocols
WB protocol for TDP-43 (human specific) antibody 60019-2-IgDownload protocol
IHC protocol for TDP-43 (human specific) antibody 60019-2-IgDownload protocol
IP protocol for TDP-43 (human specific) antibody 60019-2-IgDownload protocol
FC protocol for TDP-43 (human specific) antibody 60019-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Neurosci

Leukocyte Derived Microvesicles as Disease Progression Biomarkers in Slow Progressing Amyotrophic Lateral Sclerosis Patients.

Authors - Daisy Sproviero
humanWB

Dis Model Mech

Amyotrophic lateral sclerosis mutant TDP-43 may cause synaptic dysfunction through altered dendritic spine function.

Authors - Tongcui Jiang
humanWB

Cell Mol Life Sci

Phosphorylated and aggregated TDP-43 with seeding properties are induced upon mutant Huntingtin (mHtt) polyglutamine expression in human cellular models.

Authors - Laurent Coudert
humanWB

Front Aging Neurosci

Early Cognitive/Social Deficits and Late Motor Phenotype in Conditional Wild-Type TDP-43 Transgenic Mice.

Authors - Julio A Alfieri
humanIF

Neuropathol Appl Neurobiol

The role of lysosomes and autophagosomes in Frontotemporal Lobar Degeneration.

Authors - Hamish Dc Bain
humanIF

Acta Neuropathol Commun

RBM45 associates with nuclear stress bodies and forms nuclear inclusions during chronic cellular stress and in neurodegenerative diseases.

Authors - Mahlon Collins

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

David (Verified Customer) (01-13-2020)

Great antibody for several applications. Works nicely for immunoblot and immunofluourescence. Can also be used for RNA immunoprecipitation.

  • Applications: Western Blot,Immunofluorescence,Immunoprecipitation,
  • Primary Antibody Dilution: 1:500 (IF); 1:1000 (WB); 30µg (RIP)
  • Cell Tissue Type: SH-SY5Y