|Positive WB detected in||MCF-7 cells|
|Positive IP detected in||K-562 cells|
|Positive IHC detected in||human brain(FTLD-U) tissue, human pancreas cancer tissue, human gliomas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:5000-1:100000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:50000|
|Sample-dependent, check data in validation data gallery|
60019-2-Ig targets TDP-43 (human specific) in WB, IP, IHC, FC, CoIP, ELISA applications and shows reactivity with human samples.
|Host / Isotype||Mouse / IgG1|
|Full Name||TAR DNA binding protein|
|Calculated molecular weight||43 kDa|
|Observed molecular weight||43 kDa|
|GenBank accession number||BC001487|
|Gene ID (NCBI)||23435|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Transactivation response (TAR) DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43) was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659,19823856, 21666678, 22193176). 60019-2-Ig is a mouse monoclonal antibody recognizing the cleavage product of 20-30 kDa in addition to the native and phosphorylated forms of TDP-43. Immunohistochemical analyses of TDP-43 using this antibody detect both normal diffuse nuclear staining and insoluble inclusions in pathologic tissues. Notably this antibody only recognizes human TDP-43 but not reacts with mouse or rat TDP-43.
|Product Specific Protocols|
|WB protocol for TDP-43 (human specific) antibody 60019-2-Ig||Download protocol|
|IHC protocol for TDP-43 (human specific) antibody 60019-2-Ig||Download protocol|
|IP protocol for TDP-43 (human specific) antibody 60019-2-Ig||Download protocol|
|FC protocol for TDP-43 (human specific) antibody 60019-2-Ig||Download protocol|
|Click here to view our Standard Protocols|
Leukocyte Derived Microvesicles as Disease Progression Biomarkers in Slow Progressing Amyotrophic Lateral Sclerosis Patients.
Dis Model Mech
Amyotrophic lateral sclerosis mutant TDP-43 may cause synaptic dysfunction through altered dendritic spine function.
Cell Mol Life Sci
Phosphorylated and aggregated TDP-43 with seeding properties are induced upon mutant Huntingtin (mHtt) polyglutamine expression in human cellular models.
Front Aging Neurosci
Early Cognitive/Social Deficits and Late Motor Phenotype in Conditional Wild-Type TDP-43 Transgenic Mice.
Neuropathol Appl Neurobiol
The role of lysosomes and autophagosomes in Frontotemporal Lobar Degeneration.
Acta Neuropathol Commun
RBM45 associates with nuclear stress bodies and forms nuclear inclusions during chronic cellular stress and in neurodegenerative diseases.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
David (Verified Customer) (01-13-2020)
Great antibody for several applications. Works nicely for immunoblot and immunofluourescence. Can also be used for RNA immunoprecipitation.