Back

TDP-43 (human specific) Antibody 39 Publications

Mouse Monoclonal| Catalog number: 60019-2-Ig |CloneNo.: 6H6E12

  • Print page
  • Download PDF

-_-

Freight/Packing

Con: 160 μg/150 μl

Choose size:

Please visit your regions distributor:

Request quote for
bulk/custom formulations

Related Primary Antibodies
Special offer: Add control/tag antibody for $99
Load more
Tested Applications
Applications:
ELISA, FC, IF, IHC, IP, WB
Application not tested? Please see our Guarantee


Images See all

60019-2-Ig;human brain(FTLD-U) tissue

40X of FTLD-U case stained by 10782-2-AP and 60019-2-Ig, showing dystrophic neurites. (Figs were provided by Linda K. Kwong)

60019-2-Ig;MCF-7 cells

Various cells were subjected to SDS PAGE followed by western blot with 60019-2-Ig (TDP-43 (human specific) antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours

60019-2-Ig;K-562 cells

IP Result of anti-TDP-43 (human specific) (IP:60019-2-Ig, 5ug; Detection:60019-2-Ig 1:1000) with K-562 cells lysate 1720ug.

60019-2-Ig;MCF-7 cells

1X10^6 MCF-7 cells were stained with 0.2ug TDP-43 (human specific) antibody (60019-2-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1500.

Species specificity:
human
Positive WB detected in:
MCF-7 cells
Positive IP detected in:
K-562 cells
Positive IHC detected in:
human brain(FTLD-U) tissue, human pancreas cancer tissue, human brain tissue, human pancreas tissue, human gliomas tissue
Positive FC detected in:
MCF-7 cells
Recommended dilution:
WB : 1:5000-1:100000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:50-1:50000

Product Information

Source:
Mouse
Purification method:
Caprylic acid/ammonium sulfate precipitation
Isotype:
IgG1
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
TDP-43 (human specific) fusion protein Ag1231
Full name:
TAR DNA binding protein
Calculated molecular weight:
43 kDa
Observed molecular weight:
43 kDa
GenBank accession number:
BC001487
Gene ID (NCBI):
23435
Gene symbol
TARDBP
Synonyms
ALS10, TAR DNA binding protein, TAR DNA binding protein 43, TARDBP, TDP 43, TDP43
Background

Transactivation response (TAR) DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43) was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). 60019-2-Ig is a mouse monoclonal antibody recognizing the cleavage product of 20-30 kDa in addition to the native and phosphorylated forms of TDP-43. Immunohistochemical analyses of TDP-43 using this antibody detect both normal diffuse nuclear staining and insoluble inclusions in pathologic tissues. The epitope of 60019-2-Ig has been determined to locate at residues 203-209 (TEDELRE) by Hiroshi Tsuji et al. (2012), which is involved in the formation of pathologic TDP-43 aggregation. Notably this antibody only recognizes human TDP-43 but not reacts with mouse or rat TDP-43.


Back
to top