TDP-43 (human specific) Antibody 39 Publications

Mouse Monoclonal| Catalog number: 60019-2-Ig |CloneNo.: 6H6E12

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Freight/Packing

Con: 160 μg/150 μl

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Species specificity:
human

Positive WB detected in:
MCF-7 cells

Positive IP detected in:
K-562 cells

Positive IHC detected in:
human brain(FTLD-U) tissue, human gliomas tissue, human pancreas cancer tissue

Positive FC detected in:
MCF-7 cells

Recommended dilution:
WB : 1:5000-1:100000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:50-1:50000

Product Information


Source:
Mouse

Purification method:
Caprylic acid/ammonium sulfate precipitation

Isotype:
IgG1

Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
TAR DNA binding protein

Calculated molecular weight:
43 kDa

Observed molecular weight:
43 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
TARDBP

Synonyms
ALS10, TAR DNA binding protein, TAR DNA binding protein 43, TARDBP, TDP 43, TDP43
Background

Transactivation response (TAR) DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43) was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (17023659,19823856,21666678,22193176). 60019-2-Ig is a mouse monoclonal antibody recognizing the cleavage product of 20-30 kDa in addition to the native and phosphorylated forms of TDP-43. Immunohistochemical analyses of TDP-43 using this antibody detect both normal diffuse nuclear staining and insoluble inclusions in pathologic tissues. The epitope of 60019-2-Ig has been determined to locate at residues 203-209 (TEDELRE) by Hiroshi Tsuji et al. (2012), which is involved in the formation of pathologic TDP-43 aggregation. Notably this antibody only recognizes human TDP-43 but not reacts with mouse or rat TDP-43.


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