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  • KD/KO Validated

p115, USO1 Polyclonal antibody

p115, USO1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13509-1-AP

Synonyms

P115, Protein USO1 homolog, TAP, USO1, VDP, Vesicle docking protein



Tested Applications

Positive WB detected inHepG2 cells, HEK-293 cells, human brain tissue, mouse testis tissue, mouse thymus tissue, SH-SY5Y cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

13509-1-AP targets p115, USO1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen p115, USO1 fusion protein Ag4431
Full Name USO1 homolog, vesicle docking protein (yeast)
Calculated molecular weight 961 aa, 115 kDa
Observed molecular weight 115 kDa
GenBank accession numberBC032654
Gene symbol USO1
Gene ID (NCBI) 8615
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

p115, also known as USO1, TAP (transcytosis-associated protein) or VDP (vesicle docking protein) is a general vesicular transport factor and plays an important role at different steps of vesicular transport. It is a 962-residue peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase (PMID: 9478999). p115 forms stable homodimers (PMID: 19247479). Rab1 recruits p115 to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where p115 interacts directly with a select set of SNARE proteins (PMID: 10903204). p115 is required for intra-Golgi transport, and also functions in endoplasmic reticulum to Golgi trafficking, Golgi biogenesis and exocytotic transport (PMID: 19247479).

Protocols

Product Specific Protocols
WB protocol for p115, USO1 antibody 13509-1-APDownload protocol
IHC protocol for p115, USO1 antibody 13509-1-APDownload protocol
IF protocol for p115, USO1 antibody 13509-1-APDownload protocol
IP protocol for p115, USO1 antibody 13509-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

J Cell Sci

GM130 and p115 play a key role in the organisation of the early secretory pathway during skeletal muscle differentiation.

Authors - Emiliana Giacomello
  • KD Validated
humanIF

Bioorg Med Chem

Inhibition of diverse opportunistic viruses by structurally optimized retrograde trafficking inhibitors.

Authors - Dhimant Desai
mouseIF

J Cell Sci

The Arf GEF GBF1 and Arf4 synergize with the sensory receptor cargo, rhodopsin, to regulate ciliary membrane trafficking.

Authors - Jing Wang
mouseWB,IF

Front Mol Neurosci

Herpes Simplex Virus 1-Induced Blood-Brain Barrier Damage Involves Apoptosis Associated With GM130-Mediated Golgi Stress.

Authors - Qiang He
mouseWB,IF

PLoS One

Golgi disruption and early embryonic lethality in mice lacking USO1.

Authors - Kim Susie S
humanWB

FASEB J

Small GTPase Rab1B is associated with ATG9A vesicles and regulates autophagosome formation.

Authors - Soichiro Kakuta