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  • KD/KO Validated

Beta Catenin Polyclonal antibody

Beta Catenin Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig and More (5)

Applications

WB, IP, IHC, IF, chIP, ELISA

Conjugate

Unconjugated

Cat no : 51067-2-AP

Synonyms

b cat, Beta catenin, beta-Catenin, Catenin beta 1, CTNNB, CTNNB1, PRO2286, β catenin, β-catenin



Tested Applications

Positive WB detected inC6 cells, NIH/3T3 cells, A431 cells, human placenta tissue, HeLa cells, HEK-293 cells, mouse liver tissue, mouse lung tissue, SH-SY5Y cells, MCF-7 cells, rat liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman colon cancer tissue, human liver cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

51067-2-AP targets Beta Catenin in WB, IP, IHC, IF, chIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivity canine, cow, goat, hamster, human, mouse, pig, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name catenin (cadherin-associated protein), beta 1, 88kDa
Calculated molecular weight 781 aa, 86 kDa
Observed molecular weight 92 kDa
GenBank accession numberBC058926
Gene symbol CTNNB1
Gene ID (NCBI) 1499
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin is a 92-kDa protein, originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)

Protocols

Product Specific Protocols
WB protocol for Beta Catenin antibody 51067-2-APDownload protocol
IHC protocol for Beta Catenin antibody 51067-2-APDownload protocol
IF protocol for Beta Catenin antibody 51067-2-APDownload protocol
IP protocol for Beta Catenin antibody 51067-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

EBioMedicine

Targeting glutaminase 1 attenuates stemness properties in hepatocellular carcinoma by increasing reactive oxygen species and suppressing Wnt/beta-catenin pathway.

Authors - Binghua Li
humanWB

Aging (Albany NY)

A CREB1/miR-433 reciprocal feedback loop modulates proliferation and metastasis in colorectal cancer.

Authors - Li Yan
human,mouseWB

Molecules

Secalonic Acid-F, a Novel Mycotoxin, Represses the Progression of Hepatocellular Carcinoma via MARCH1 Regulation of the PI3K/AKT/β-catenin Signaling Pathway.

Authors - Lulu Xie
humanWB

In Vitro Cell Dev Biol Anim

A1CF-Axin2 signal axis regulates apoptosis and migration in Wilms tumor-derived cells through Wnt/β-catenin pathway.

Authors - Dongsheng Ni
humanWB

Front Oncol

Scorpion Venom Analgesic Peptide, BmK AGAP Inhibits Stemness, and Epithelial-Mesenchymal Transition by Down-Regulating PTX3 in Breast Cancer.

Authors - Sylvanus Kampo
humanWB

FASEB J

PMID: 30844312

Authors - Tengyu Ko

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Alzbeta (Verified Customer) (03-13-2019)

This antibody was used for IF staining of beta-catenin in primary neonatal rat cardiomyocyte culture. The antibody worked well after both ice cold-methanol or 4% PFA fixation, followed by the 3%BSA block. The incubation with primary (b-catenin) and secondary antibody (aRabbit-Alexa Fluor 488 Plus) lasted 1 hr at room temperature.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Primary rat neonatal cardiomyocytes
Beta Catenin Antibody Immunofluorescence, validation (1:100 dilution) in Primary rat neonatal cardiomyocytes (Cat no:51067-2-AP)