CL488-conjugated beta Actin Antibody

beta Actin Monoclonal Antibody for IF, ELISA

Host / Isotype

Mouse / IgM

Reactivity

human, mouse, rat, pig, yeast, plant, Zebrafish

Applications

IF, ELISA

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

7D2C10

Cat no : CL488-60008

Synonyms

ACTB, Actin, beta Actin



Tested Applications

Positive IF detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 60008-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-60008 targets CL488-conjugated beta Actin in IF, ELISA applications and shows reactivity with human, mouse, rat, pig, yeast, plant, Zebrafish samples.

Tested Reactivity human, mouse, rat, pig, yeast, plant, Zebrafish
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen beta Actin fusion protein Ag0297
Full Name actin, beta
Calculated molecular weight 375aa,42 kDa
Observed molecular weight
GenBank accession number BC002409
Gene symbol ACTB
Gene ID (NCBI) 60
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm/515 nm
Form Liquid
Purification Method Caprylic acid/ammonium sulfate precipitation
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody is conjugated with CL488(Ex/Em 488 nm/515 nm), and can recognize all the actins.

Protocols

Product Specific Protocols
IF protocol for CL488-conjugated beta Actin antibody CL488-60008Download protocol
Standard Protocols
Click here to view our Standard Protocols