Tested Applications
| Positive IF/ICC detected in | Live vaccines of TGEV and PEDV-infected Vero cells, Transfected Vero |
| Positive FC (Intra) detected in | Transfected Vero |
| Positive Dot Blot detected in | N/A, Poly(I:C) |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| DOT BLOT | DOT BLOT : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85780-2-RR targets dsRNA in IF/ICC, FC (Intra), Dot Blot, ELISA applications and shows reactivity with n/a samples.
| Tested Reactivity | n/a |
| Cited Reactivity | human, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
dsRNA Predict reactive species |
| Full Name | dsRNA |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_3744367 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
dsRNA is formed by the base pairing of two RNA strands, creating a double helix structure similar to DNA, but with some differences. dsRNA can be generated through various mechanisms. In the context of viral infections, it can be derived from the genomes of dsRNA viruses, replication intermediates of single-stranded RNA viruses, or bidirectional transcription of certain DNA viruses. Additionally, aberrant splicing or transcriptional processes may lead to the formation of dsRNA structures. dsRNA is recognized as a pathogen-associated molecular pattern (PAMP) by the immune system. It can trigger a series of immune responses in cells, including the activation of antiviral innate immune pathways.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for dsRNA antibody 85780-2-RR | Download protocol |
| IF protocol for dsRNA antibody 85780-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The dsRNA Recombinant monoclonal antibody (250708A2), catalog number 85780-2-RR, has 4 total citations. These appear in Stem Cell Research & Therapy, Virulence, International Journal of Molecular Sciences, and Veterinary Research. The associated research fields include stem cell biology and mitophagy, viral replication (enterovirus 71, PRRSV, and bovine coronavirus), ROS-mediated antiviral mechanisms, and PANoptosis.
| Species | Application | Title |
|---|---|---|
Stem Cell Res Ther DS96432529 enhances osteogenic differentiation and mitigates inflammatory damage in periodontal ligament stem cells involving mitophagy-related processes. | ||
Virulence Calycosin targets the MAPK-EGR1 signaling axis to inhibit enterovirus 71 replication in cells and human colonic organoids. | ||
Vet Res Neu1 inhibition restrains BCoV replication and modulates ZBP1-dependent PANoptosis. |
