AGO2 Monoklonaler Antikörper

AGO2 Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, RIP, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

CloneNo.

4E2B2

Kat-Nr. : 66720-1-Ig

Synonyme

AGO2, Argonaute2, eIF 2C 2, eIF2C 2, EIF2C2, hAgo2, PAZ Piwi domain protein, PPD, Protein argonaute 2, Protein slicer, Q10

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Galerie der Validierungsdaten

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  WB analysis using 66720-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66720-1-Ig (AGO2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 66720-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66720-1-Ig (AGO2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 66720-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66720-1-Ig (AGO2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 66720-1-Ig

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 66720-1-Ig (AGO2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 66720-1-Ig

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 66720-1-Ig (AGO2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, humanes Urothelkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IP: See 2 publications below
• RIP: See 3 publications below

Produktinformation

66720-1-Ig bindet in WB, RIP, IP, IHC, ELISA AGO2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: AGO2 fusion protein Ag17723
• Vollständiger Name: eukaryotic translation initiation factor 2C, 2
• Berechnetes Molekulargewicht: 97 kDa
• Beobachtetes Molekulargewicht: 97 kDa
• GenBank-Zugangsnummer: BC007633
• Gene symbol: EIF2C2
• Gene ID (NCBI): 27161
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EIF2C2, also named as AGO2, belongs to the argonaute family and Ago subfamily. It is required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include EIF2C2/AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). EIF2C2 may inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. It also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. EIF2C2 can also upregulate the translation of specific mRNAs under certain growth conditions.

Publikationen

Species	Application	Title
human	IP	Front Oncol; Overexpressed Tumor Suppressor Exosomal miR-15a-5p in Cancer Cells Inhibits PD1 Expression in CD8+T Cells and Suppresses the Hepatocellular Carcinoma Progression.; Authors - Hong-Yu Zhang
	IP	Cell Signal; CircZNF208 enhances the sensitivity to X-rays instead of carbon-ions through the miR-7-5p /SNCA signal axis in non-small-cell lung cancer cells.; Authors - Bingtao Liu
human	RIP	Biosci Rep; LncRNA HOXA-AS2 promotes the progression of prostate cancer via targeting miR-509-3p/PBX3 axis.; Authors - Shangwen Xiao
human	RIP	J Bone Miner Metab; Regulation of circADAMTS6-miR-324-5p-PIK3R3 ceRNA pathway may be a novel mechanism of IL-1β-induced osteoarthritic chondrocytes.; Authors - Lanjuan Shen
human	RIP	J Orthop Surg Res; Circ-FOXM1 promotes the proliferation, migration and EMT process of osteosarcoma cells through FOXM1-mediated Wnt pathway activation.; Authors - Hao Zhang