AKT2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

AKT2 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

14

Rezensionen (1)

Kat-Nr. : 17609-1-AP

Synonyme

AKT2, PKB beta, PKBB, PKBBETA, PRKBB, Protein kinase Akt 2, Protein kinase B beta, RAC BETA, RAC PK beta

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Galerie der Validierungsdaten

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  WB analysis of NIH/3T3 using 17609-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 17609-1-AP (AKT2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 17609-1-AP

  IP Result of anti-AKT2 (IP:17609-1-AP, 4ug; Detection:17609-1-AP 1:500) with HepG2 cells lysate 1600ug.

• 

  IHC staining of human liver using 17609-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17609-1-AP (AKT2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 17609-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17609-1-AP (AKT2 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human breast cancer using 17609-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 17609-1-AP (AKT2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human breast cancer using 17609-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 17609-1-AP (AKT2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver cancer using 17609-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 17609-1-AP (AKT2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver cancer using 17609-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 17609-1-AP (AKT2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary tumor using 17609-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 17609-1-AP (AKT2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary tumor using 17609-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 17609-1-AP (AKT2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 17609-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17609-1-AP (AKT2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 17609-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17609-1-AP (AKT2 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Leberkarzinomgewebe, humanes Mammakarzinomgewebe, humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 13 publications below
• IHC: See 2 publications below

Produktinformation

17609-1-AP bindet in WB, IP, IHC, ELISA AKT2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AKT2 fusion protein Ag11770
• Vollständiger Name: v-akt murine thymoma viral oncogene homolog 2
• Berechnetes Molekulargewicht: 481 aa, 56 kDa
• Beobachtetes Molekulargewicht: 56 kDa
• GenBank-Zugangsnummer: BC063421
• Gene symbol: AKT2
• Gene ID (NCBI): 208
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AKT2 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis and their activation has been observed in a wide variety of cancers. AKT2 is mainly involved in cancer cell survival, apoptosis inhibition, migration and invasion(PMID:21979951). Defects in AKT2 are a cause of susceptibility to breast cancer (BC). AKT2 promotes metastasis of tumor cells without affecting the latency of tumor development. And defects in AKT2 are a cause of non-insulin-dependent diabetes mellitus (NIDDM) and hypoinsulinemic hypoglycemia with hemihypertrophy (HIHGHH). The full length protein has four glycosylation sites.

Publikationen

Species	Application	Title
mouse	WB	EMBO J; Asparagine reinforces mTORC1 signaling to boost thermogenesis and glycolysis in adipose tissues.; Authors - Yingjiang Xu
human	WB,IHC	Respir Res; AKT2 drives cancer progression and is negatively modulated by miR-124 in human lung adenocarcinoma.; Authors - Ting Liu ; KD Validated
human	WB	Oncotarget; Estrogen regulates miRNA expression: implication of estrogen receptor and miR-124/AKT2 in tumor growth and angiogenesis.; Authors - Cheng-Fei Jiang
rat	WB	Food Funct; l-Theanine regulates glucose, lipid, and protein metabolism via insulin and AMP-activated protein kinase signaling pathways; Authors - Ling Lin
human	WB	Front Cell Dev Biol; miR-29b-3p Increases Radiosensitivity in Stemness Cancer Cells via Modulating Oncogenes Axis; Authors - Dong Pan
human	WB	Front Cell Dev Biol; Regulation of MicroRNA-497-Targeting AKT2 Influences Tumor Growth and Chemoresistance to Cisplatin in Lung Cancer.; Authors - Lin Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tom (Verified Customer) (11-03-2020)

Antibody diluted at 1:500 in blocking solution (5% BSA in TBS) and incubated overnight at 4 degrees.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HEK293T

AKT2 Antibody Western Blot validation (1:500 dilution) in HEK293T (Cat no:17609-1-AP)