CD107a / LAMP1 Rekombinanter Antikörper

CD107a / LAMP1 Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

Unkonjugiert

CloneNo.

H4A3

Kat-Nr. : 65519-1-MR

Synonyme

CD107a, LAMP1, CD107 antigen-like family member A, CD107a/LAMP1, H4A3

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  FC experiment of HeLa using 65519-1-MR

  1x10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human CD107a Mouse Recombinant Antibody (65519-1-MR, Clone:H4A3) or Mouse IgG1 Isotype Control (65124-1-Ig, Clone: MOPC-21) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) (SA00014-8). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• 

  FC experiment of HeLa using 65519-1-MR

  1x10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human CD107a Mouse Recombinant Antibody (65519-1-PBS, Clone:H4A3) or Mouse IgG1 Isotype Control (65124-1-Ig, Clone: MOPC-21) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) (SA00014-8). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• 

  FC experiment of HeLa using 65519-1-MR

  1x10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human CD107a Mouse Recombinant Antibody (65519-1-PBS, Clone:H4A3) (left and red) or 0.2 ug Anti-Human CD107a Mouse Monoclonal Antibody (65051-1-Ig, Clone:H4A3) (center and black dashed) or Mouse IgG1 Isotype Control (65124-1-Ig, Clone: MOPC-21) (blue) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) (SA00014-8). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

65519-1-MR bindet in FC (Intra) CD107a / LAMP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Humane adhärente periphere Blutzellen
• Vollständiger Name: lysosomal-associated membrane protein 1
• Berechnetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC006345
• Gene symbol: LAMP1
• Gene ID (NCBI): 3916
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).