CD107a / LAMP1 Monoklonaler Antikörper

CD107a / LAMP1 Monoklonal Antikörper für FC (Intra)

Wirt / Isotyp

Ratte / IgG2a, kappa

Getestete Reaktivität

Maus

Anwendung

FC (Intra)

Konjugation

FITC Plus Fluorescent Dye

Publikationen(3)

CloneNo.

1D4B

Kat-Nr. : FITC-65050

Synonyme

CD107a, Lamp1, Lamp 1, 1D4B

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  FC experiment of NIH/3T3 using FITC-65050

  1x10^6 NIH/3T3 cells were intracellularly stained with 1 ug FITC Anti-Mouse CD107a / LAMP1 (FITC-65050, Clone:1D4B) (red) or 1 ug FITC Rat IgG2a Isotype Control (2A3) (FITC-65209, Clone: 2A3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): NIH/3T3-Zellen

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• FC: See 3 publications below

Produktinformation

FITC-65050 bindet in FC (Intra) CD107a / LAMP1 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2a, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Plasmamembranfraktion einer Mausembryo-NIH/3T3-Zelllinie
• Vollständiger Name: lysosomal-associated membrane protein 1
• GenBank-Zugangsnummer: BC006785
• Gene symbol: CD107a
• Gene ID (NCBI): 16783
• Konjugation: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).

Publikationen

Species	Application	Title
mouse	FC	Immunity; STING activation in platelets aggravates septic thrombosis by enhancing platelet activation and granule secretion; Authors - Mina Yang
mouse	FC	Front Immunol; ELK4 exerts opposite roles in cytokine/chemokine production and degranulation in activated mast cells; Authors - Yuji Huang
mouse	FC	Nat Nanotechnol; A bispecific nanosystem activates endogenous natural killer cells in the bone marrow for haematologic malignancies therapy; Authors - Yanqin Zhang