CD11b/c Monoklonaler Antikörper

CD11b/c Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG2a, kappa

Getestete Reaktivität

Ratte und mehr (1)

Anwendung

IF, FC

Konjugation

CoraLite® Plus 647 Fluorescent Dye

Publikationen(1)

CloneNo.

OX42

Kat-Nr. : CL647-65229

Synonyme

CD11b, integrin alpha M, Itgam, OX42

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• 

  FC experiment of wistar rat splenocytes using CL647-65229

  1X10^6 Wistar rat splenocytes were surface stained with 0.5 ug CoraLite® Plus 647 Anti-Rat CD11b/c (CL647-65229, Clone: OX42) or 5 ul Control Antibody. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Wistar rat splenocytes

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

CL647-65229 bindet in IF, FC CD11b/c und zeigt Reaktivität mit Ratten

• Getestete Reaktivität: Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Rat peritoneal macrophages
• Vollständiger Name: integrin alpha M
• Berechnetes Molekulargewicht: 127 kDa
• GenBank-Zugangsnummer: NM_012711
• Gene symbol: CD11b
• Gene ID (NCBI): 25021
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b (integrin alpha M or CR3A) and CD11c (integrin alpha X) are members of the integrin alpha chain family and can form α/β heterodimers with CD18 (integrin β2). CD11b/CD18 is a receptor for the complement protein fragment C3bi, and is also a receptor for fibrinogen, factor X, and ICAM1 (PMID: 2971974; 15485828). CD11c/CD18 acts as a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis (PMID: 1671533). The OX-42 antibody recognizes a common epitope shared between CD11b and CD11c. It binds to C3bi receptor and is known to block C3bi binding (PMID: 3512425; 8449218).

Publikationen

Species	Application	Title
human	IF	Cell Biosci; Tie2-expressing monocytes/macrophages promote angiogenesis in chronically ischaemic brain tissue; Authors - Chuyang Tai