CoraLite® Plus 750 Anti-Rat CD11b/c (OX42) Mouse IgG2a Recombinant Antibody

CD11b/c Uni-rAb® Recombinant Antibody for FC

Cat No. CL750-65606
Clone No.OX42

Host / Isotype

Mouse / IgG2a

Reactivity

rat

Applications

FC

CD11b, integrin alpha M, Itgam, OX42

Formulation:  PBS and Azide
PBS and Azide
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive FC detected inrat bone marrow cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL750-65606 targets CD11b/c in FC applications and shows reactivity with rat samples.

Tested Reactivity rat
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen

fusion protein

Predict reactive species
Full Name integrin alpha M
Calculated Molecular Weight127 kDa
GenBank Accession NumberNM_012711
Gene Symbol CD11b
Gene ID (NCBI) 25021
RRIDAB_3673713
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission Maxima Wavelengths755 nm / 780 nm
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9JI30
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b (integrin alpha M or CR3A) and CD11c (integrin alpha X) are members of the integrin alpha chain family and can form α/β heterodimers with CD18 (integrin β2). CD11b/CD18 is a receptor for the complement protein fragment C3bi, and is also a receptor for fibrinogen, factor X, and ICAM1 (PMID: 2971974; 15485828). CD11c/CD18 acts as a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis (PMID: 1671533). The OX-42 antibody recognizes a common epitope shared between CD11b and CD11c. It binds to C3bi receptor and is known to block C3bi binding (PMID: 3512425; 8449218).

Protocols

Product Specific Protocols
FC protocol for CL Plus 750 CD11b/c antibody CL750-65606Download protocol
Standard Protocols
Click here to view our Standard Protocols
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