CoraLite®594 Anti-Rat CD11b/c (OX42) Mouse IgG2a Recombinant Antibody

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  FC experiment of rat bone marrow cells using CL594-65606

  1x10^6 rat bone marrow cells were surface stained with 0.25 ug CoraLite®594 Anti-Rat CD11b/c (OX42) Mouse IgG2a Recombinant Antibody (CL594-65606, Clone:OX42), or CoraLite®594 Mouse IgG2a Isotype Control (C1.18.4) (CL594-65208, Clone: C1.18.4). Cells were not fixed.

• 

  FC experiment of rat bone marrow cells using CL594-65606

  1x10^6 rat bone marrow cells were surface stained with 0.25 ug CoraLite®594 Anti-Rat CD11b/c (OX42) Mouse IgG2a Recombinant Antibody (CL594-65606, Clone:OX42) (red), or CoraLite®594 Mouse IgG2a Isotype Control (C1.18.4) (CL594-65208, Clone: C1.18.4) (blue). Cells were not fixed.

CD11b/c Uni-rAb^® Recombinant Antibody for FC

Cat No. CL594-65606

Clone No.OX42

Host / Isotype

Mouse / IgG2a

Reactivity

rat

Applications

FC

Synonyms

CD11b, integrin alpha M, Itgam, OX42

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Tested Applications

• Positive FC detected in: rat bone marrow cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-65606 targets CD11b/c in FC applications and shows reactivity with rat samples.

• Tested Reactivity: rat
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Rat peritoneal macrophages

• Full Name: integrin alpha M
• Calculated Molecular Weight: 127 kDa
• GenBank Accession Number: NM_012711
• Gene Symbol: CD11b
• Gene ID (NCBI): 25021
• RRID: AB_3673493
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9JI30
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b (integrin alpha M or CR3A) and CD11c (integrin alpha X) are members of the integrin alpha chain family and can form α/β heterodimers with CD18 (integrin β2). CD11b/CD18 is a receptor for the complement protein fragment C3bi, and is also a receptor for fibrinogen, factor X, and ICAM1 (PMID: 2971974; 15485828). CD11c/CD18 acts as a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis (PMID: 1671533). The OX-42 antibody recognizes a common epitope shared between CD11b and CD11c. It binds to C3bi receptor and is known to block C3bi binding (PMID: 3512425; 8449218).