LAG-3/CD223 Monoklonaler Antikörper

LAG-3/CD223 Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG1, kappa

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

C9B7W

Kat-Nr. : CL647-65098

Synonyme

CD223, LAG-3, Ly66, Lag3, LAG 3

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  FC experiment of BALB/c mouse splenocytes using CL647-65098

  1X10^6 Con-A stimulated BALB/c mouse splenocytes were surface stained with CoraLite®488 Anti-Mouse CD3 (CL488-65077, Clone: 17A2) and 0.5 ug CoraLite® Plus 647 Anti-Mouse CD223 (CL647-65098, Clone: C9B7W) or 0.5 ug CoraLite® Plus 647-conjugated Rat IgG1 isotype control. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Con-A stimulated BALB/c mouse splenocytes

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL647-65098 bindet in FC LAG-3/CD223 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Murines CD223-Ig-Fusionsprotein
• Vollständiger Name: lymphocyte-activation gene 3
• GenBank-Zugangsnummer: BC120591
• Gene symbol: CD223
• Gene ID (NCBI): 16768
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Lymphocyte activation gene 3 protein (LAG-3), also known as CD223, is a type I transmembrane protein belonging to the immunoglobulin superfamily (PMID: 1692078). It is a CD4-related molecule that binds MHC class II with high affinity (PMID: 1692078; 9159144). LAG-3 is expressed on activated T and NK cells. Besides, natural CD4(+)CD25(+) Tregs express LAG-3 upon activation (PMID: 15485628). LAG-3 negatively regulates T-cell function and homeostasis, and contributes to the suppressor activity of Tregs (PMID: 14644131; 15485628).