CD25/IL-2RA Monoklonaler Antikörper

CD25/IL-2RA Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG1, lambda

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

FITC Plus Fluorescent Dye

Publikationen(4)

CloneNo.

PC61.5

Kat-Nr. : FITC-65137

Synonyme

CD25, Il-2ra, Il2ra, IL-2R subunit alpha, Il2r

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  FC experiment of mouse splenocytes using FITC-65137

  1x10^6 untreated or anti-CD3/CD28 treated (2 days) mouse splenocytes were surface stained with CoraLite® Plus 647 Anti-Mouse CD4 and 0.25 ug FITC Plus Anti-Mouse CD25 (PC61.5) (FITC-65137, Clone: PC61.5). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using FITC-65137

  1x10^6 anti-CD3/CD28 treated (2 days) mouse splenocytes were surface stained with 0.25 ug FITC Plus Anti-Mouse CD25 (PC61.5) (FITC-65137, Clone: PC61.5) (red) or 0.25 ug FITC Plus Rat IgG1 Isotype Control (HRPN) (FITC-65212, Clone: HRPN) (blue). 1x10^6 untreated mouse splenocytes were surface stained with 0.25 ug FITC Plus Anti-Mouse CD25 (PC61.5) (FITC-65137, Clone: PC61.5) (black, dashed). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: anti-CD3/CD28 treated mouse splenocytes

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• FC: See 2 publications below

Produktinformation

FITC-65137 bindet in FC CD25/IL-2RA und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG1, lambda
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: interleukin 2 receptor, alpha chain
• GenBank-Zugangsnummer: BC114437
• Gene symbol: CD25
• Gene ID (NCBI): 16184
• Konjugation: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+Foxp3+ Treg cells (PMID: 22585674).

Publikationen

Species	Application	Title
	FC	Oncogene; ZG16 enhances the maturation of dendritic cells via induction of CD40 and contributes to the antitumor immunity in pancreatic cancer; Authors - Hui Meng
mouse	FC	Phytomedicine; Multi-material basis and multi-mechanisms of the Dahuang Zhechong pill for regulating Treg/Th1 balance in hepatocellular carcinoma.; Authors - Li Wu
		Cell Rep; Podocyte SIRPα reduction aggravates lupus nephritis via promoting T cell inflammatory responses; Authors - Bin Qian
Mouse		BMC Pharmacol Toxicol; Blocking group 2 innate lymphoid cell activation and macrophage M2 polarization: potential therapeutic mechanisms in ovalbumin-induced allergic asthma by calycosin; Authors - Chunyan Tian