Biotin Anti-Mouse CD25/IL-2RA Rabbit Recombinant Antibody

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  FC experiment of mouse splenocytes using Biotin-98288

  1x10^6 untreated or anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug Biotin Anti-Mouse CD25/IL-2RA Rabbit RecAb (Biotin-98288, Clone: 242494H5) and CoraLite®488-conjugated streptavidin or unstained. Cells were co-stained with APC Anti-Mouse CD4. Cells were not fixed.

• 

  FC experiment of mouse splenocytes using Biotin-98288

  1x10^6 anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug Biotin Anti-Mouse CD25/IL-2RA Rabbit RecAb (Biotin-98288, Clone: 242494H5) and CoraLite®488-conjugated streptavidin (red), or unstained (blue). 1x10^6 untreated mouse splenocytes were surface stained with 0.25 ug Biotin Anti-Mouse CD25/IL-2RA Rabbit RecAb (Biotin-98288, Clone: 242494H5) and CoraLite®488-conjugated streptavidin (black, dashed). Cells were not fixed.

CD25/IL-2RA Uni-rAb^TM Recombinant Antibody for FC

Cat No. Biotin-98288

Clone No.242494H5

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

CD25, 242494H5, IL-2 receptor subunit alpha, Il2r, IL-2R subunit alpha

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Tested Applications

• Positive FC detected in: Anti-CD3/CD28 treated mouse splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-98288 targets CD25/IL-2RA in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: interleukin 2 receptor, alpha chain
• Calculated Molecular Weight: 31 kDa
• GenBank Accession Number: NM_008367.3
• Gene Symbol: CD25
• Gene ID (NCBI): 16184
• Conjugate: Biotin
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P01590
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+Foxp3+ Treg cells and a subset of human CD4+ memory T cells (PMID: 22585674).