Collagen Type III (C-terminal) Polyklonaler Antikörper

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• KD/KO Validated

Collagen Type III (C-terminal) Polyklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(49)

Kat-Nr. : 13548-1-AP

Synonyme

Collagen Type III (C-terminal)

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  IHC staining of human hepatocirrhosis using 13548-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 13548-1-AP (Collagen Type III (C-terminal) antibody at dilution of 1:100 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 13548-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 13548-1-AP (Collagen Type III (C-terminal) antibody at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Leberzirrhosegewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 42 publications below
• IHC: See 6 publications below
• IF: See 4 publications below

Produktinformation

13548-1-AP bindet in WB, IHC, IF, ELISA Collagen Type III (C-terminal) und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Collagen Type III (C-terminal) fusion protein Ag4229
• Vollständiger Name: collagen, type III, alpha 1
• Berechnetes Molekulargewicht: 1466 aa, 139 kDa
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: BC028178
• Gene symbol: COL3A1
• Gene ID (NCBI): 1281
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Type III collagen is a fibrillar forming collagen comprising three a1(III) chains and is expressed in early embryos and throughout embryogenesis. In the adult, type III collagen is a major component of the extracellular matrix in a variety of internal organs and skin. It occurs in most soft connective tissues along with type I collagen. COL3A1 gene encodes type III procollagen. Mutations in this gene are associated with type III and IV Ehlers-Danlos syndrome, and with aortic and arterial aneurysms. (PMID: 9050868; 2445760)

Publikationen

Species	Application	Title
human	WB	Redox Biol; Silencing COX-2 blocks PDK1/TRAF4-induced AKT activation to inhibit fibrogenesis during skeletal muscle atrophy.; Authors - Hongtao Chen
mouse	IHC	Oxid Med Cell Longev; Myricetin Possesses Potential Protective Effects on Diabetic Cardiomyopathy through Inhibiting IκBα/NFκB and Enhancing Nrf2/HO-1.; Authors - Hai-Han Liao
human	WB,IF	Oncotarget; Epithelial but not stromal expression of collagen alpha-1(III) is a diagnostic and prognostic indicator of colorectal carcinoma.; Authors - Xiao-Qing Wang ; KD Validated
		Cell Death Dis; Rosmarinic acid attenuates cardiac fibrosis following long-term pressure overload via AMPKα/Smad3 signaling.; Authors - Xin Zhang
mouse,rat	WB	J Mol Cell Cardiol; Ascorbate starvation alters endoplasmic reticulum-resident enzymes in cardiac fibroblasts, priming them for increased procollagen secretion.; Authors - Randy T Cowling
mouse	WB	Sci Rep; Deletion of interleukin-6 alleviated interstitial fibrosis in streptozotocin-induced diabetic cardiomyopathy of mice through affecting TGFβ1 and miR-29 pathways.; Authors - Yang Zhang