CXCL4/PF4 Rekombinanter Antikörper

CXCL4/PF4 Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

Unkonjugiert

CloneNo.

243087D5

Kat-Nr. : 98410-2-RR

Synonyme

CXCL4, PF4, SCYB4, C X C motif chemokine 4, C-X-C motif chemokine 4

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  FC experiment of human PBMCs using 98410-2-RR

  1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human CXCL4/PF4 Rabbit RecAb (98410-2-RR, Clone: 243087D5) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Human CD14. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of human PBMCs using 98410-2-RR

  1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human CXCL4/PF4 Rabbit RecAb (98410-2-RR, Clone: 243087D5) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Monocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98410-2-RR bindet in FC (Intra) CXCL4/PF4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CXCL4/PF4 fusion protein Eg2129
• Vollständiger Name: platelet factor 4
• Berechnetes Molekulargewicht: 11 kDa
• GenBank-Zugangsnummer: NM_002619.4
• Gene symbol: PF4
• Gene ID (NCBI): 5196
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

CXCL4, also known as platelet factor 4 (PF-4), is the oldest member of the chemokine family. CXCL4 is a chemokine produced by activated platelets and immune cells involved in pathological conditions such as cancer, infections and inflammatory diseases like systemic sclerosis (SSc), rheumatoid arthritis (RA) and psoriatic arthritis (PsA), among others. CXCL4 is present in the α-granules of platelets in amounts that constitute up to 2% platelet protein mass. CXCL4 plays a determinant role in distinct physiological processes such as in hematopoiesis, angiogenesis, coagulation and modulation of immune responses. For instance, CXCL4 can prevent monocyte apoptosis and promote cell survival, induces the production of TNF and reactive oxygen species (ROS) and promotes monocyte differentiation into a unique macrophage-like phenotype.