DYKDDDDK tag Rekombinanter Antikörper

DYKDDDDK tag Rekombinant Antikörper für IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

rekombinanten Protein und mehr (2)

Anwendung

IF/ICC, IP, CoIP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(140)

CloneNo.

4K14

Rezensionen (2)

Kat-Nr. : 80010-1-RR

Synonyme

Flag, Flag tag, DDDDK, DDDDK tag, DYKDDDDK

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  IP experiment of Transfected HEK-293 using 80010-1-RR

  IP result of anti-DYKDDDDK tag (IP:80010-1-RR, 4ug; Detection:80010-1-RR 1:3000) with Transfected HEK-293 cells lysate 400 ug.

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  IF Staining of HEK-293 using 80010-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 (eGFP-DYKDDDDK transfected) cells using DYKDDDDK tag antibody (80010-1-RR, Clone: 4K14 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), eGFP (Green), DYKDDDDK (Red), DAPI (Blue).

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  IF Staining of Transfected HEK-293 using 80010-1-RR

  Immunofluorescent analysis of (4% PFA) fixed Transfected HEK-293 cells using DYKDDDDK tag antibody (80010-1-RR, Clone: 4K14 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of Transfected HEK-293 using 80010-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using 80010-1-RR (DDDDK tag antibody), at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of Transfected HEK-293 using 80010-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using 80010-1-RR (DYKDDDDK tag antibody), at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HEK-293 using 80010-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using DYKDDDDK tag antibody (80010-1-RR, Clone: 4K14 ) at dilution of 1:500 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), GFP (green).

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  FC experiment of Transfected HEK-293 using 80010-1-RR

  1x10^6 Transfected HEK-293 cells were intracellularly stained with 0.25 ug DYKDDDDK tag Recombinant antibody (Binds to FLAG® tag epitope) (80010-1-RR, Clone:4K14) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 80010-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80010-1-RR against DYKDDDDK tag were performed. The affinity constant is 83.7 pM.

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Geprüfte Anwendungen

• Erfolgreiche IP: Transfizierte HEK-293-Zellen
• Erfolgreiche Detektion in IF/ICC: Transfizierte HEK-293-Zellen, HEK-293-Zellen
• Erfolgreiche Detektion in FC (Intra): Transfizierte HEK-293-Zellen

Empfohlene Verdünnung

• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:1000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 21 publications below
• IP: See 47 publications below
• CoIP: See 16 publications below
• RIP: See 1 publications below

Produktinformation

80010-1-RR bindet in IF/ICC, IP, CoIP, RIP, ELISA DYKDDDDK tag und zeigt Reaktivität mit rekombinanten Protein

• Getestete Reaktivität: rekombinanten Protein
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: DYKDDDDK tag fusion protein Ag2329
• Vollständiger Name: DYKDDDDK tag
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DYKDDDDK Tag (Equivalent To FLAG Antibody From Sigma) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic proteins. Due to its high hydrophilic character, the DYKDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag to be accessible for antibodies. DYKDDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.

Publikationen

Species	Application	Title
		Mol Cancer; Overcoming multi-drug resistance in SCLC: a synergistic approach with venetoclax and hydroxychloroquine targeting the lncRNA LYPLAL1-DT/BCL2/BECN1 pathway; Authors - Shuxin Li
		Mol Cancer; A positive feedback circuit driven by m6A-modified circular RNA facilitates colorectal cancer liver metastasis; Authors - Kaixuan Zeng
		Cell Metab; TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis.; Authors - Dong Huang
	IP	Nat Commun; Inhibited peroxidase activity of peroxiredoxin 1 by palmitic acid exacerbates nonalcoholic steatohepatitis in male mice; Authors - Wen Yin
	IF	Nat Commun; A lung-selective delivery of mRNA encoding broadly neutralizing antibody against SARS-CoV-2 infection; Authors - Wanbo Tai
		Adv Sci (Weinh); The Hemagglutinin of Influenza A Virus Induces Ferroptosis to Facilitate Viral Replication; Authors - Aotian Ouyang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Abigail (Verified Customer) (06-07-2024)

Used for looking at scfv expression using western blot. Worked well, giving bright bands at the expected scfv length.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Brain cortex

FH

Xin (Verified Customer) (04-17-2022)

It is good to detect flag-tagged protein expressed in hek293 cells, although efficiency is not so high as 20543-1-AP.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: hek293