CoraLite® Plus 488-conjugated DYKDDDDK tag Recombinant antibody (Binds to FLAG® tag epitope)

DYKDDDDK tag Uni-rAbTM Recombinant Antibody for FC (Intra)
Cat No. CL488-80801-2
Clone No.240568A11

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein

Applications

FC (Intra)

3Flag, Ag2329, Flag, DDDDK, DDDDK tag

Formulation:  PBS and Azide
PBS and Azide
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive FC (Intra) detected inHEK-293T transfected with TDP-43-flag

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-80801-2 targets DYKDDDDK tag in FC (Intra) applications and shows reactivity with recombinant protein samples.

Tested Reactivity recombinant protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen DYKDDDDK tag fusion protein Ag2329 Predict reactive species
Full Name Flag Tag
Gene Symbol Flag Tag
Gene ID (NCBI) 8
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal DDDDK vectors provide an Ek cleavage site for removal of the fusion tag. The DDDDK peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the DDDDK peptide is more likely to be accessible to antibodies. A DDDDK-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on. This antibody is a rabbit recombinant antibody raised against 3xFlag (3xDYKDDDDKT) sequence and recognizes the (1x) and (3x)DYKDDDDK peptide and detects DDDDK-tagged proteins. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 DYKDDDDK tag antibody CL488-80801-2Download protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Fabian (Verified Customer) (04-25-2025)

I compared the antibody in question with the older Antibody (Prod.-No. 80810-1-RR) which is no longer available. In my setting, I tested sample from a purified protein complex which I am working on. I noticed that the older antibody does detect unspecific bands, while the newer antibody in question seems to be more specific. The intensities of the detected signal was comparable and similar. Please find my results attached.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
CL Plus 488 DYKDDDDK tag Antibody Western Blot validation (1:1000 dilution) in (Cat no:CL488-80801-2)
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