Desmin Monoklonaler Antikörper

Desmin Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human und mehr (2)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

8

CloneNo.

3E12C8

Kat-Nr. : 60226-1-Ig

Synonyme

CMD1I, CSM1, CSM2, DES, desmin

---

Galerie der Validierungsdaten

• 

  WB analysis using 60226-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60226-1-Ig (Desmin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 60226-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 60226-1-Ig (DES antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Human heart, Hausschwein-Herzgewebe, Hausschwein-Kolongewebe, humanes Herzgewebe, humanes Hodengewebe, humanes Plazenta-Gewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Produktinformation

60226-1-Ig bindet in WB, IHC, IF, ELISA Desmin und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Desmin fusion protein Ag9675
• Vollständiger Name: desmin
• Berechnetes Molekulargewicht: 470 aa, 54 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC032116
• Gene symbol: DES
• Gene ID (NCBI): 1674
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Desmin is the main intermediate filament protein in skeletal and cardiac muscle cells and is essential for both the structural integrity and the survival of muscle cells. As an abundant muscle-specific protein, desmin has been widely used as a marker of muscle derived tumors. Anti-desmin is also valuable in the differential diagnosis of tumors of uncertain origin.

Publikationen

Species	Application	Title
mouse	WB	Br J Pharmacol; Catalpol Alleviates Adriamycin-Induced Nephropathy by Activating the SIRT1 Signalling Pathway In Vivo and In Vitro.; Authors - Jiangnan Zhang
rat	IF	Diabetes Metab Res Rev; Gene expression profiles of rat MMECs with different glucose levels and fgl2 gene silencing.; Authors - Zhenzhong Zheng
mouse	IHC	Exp Cell Res; Effect of sarcolipin-mediated cell transdifferentiation in sarcopenia-associated skeletal muscle fibrosis.; Authors - Qiu-Nan Chen
human	IF	Exp Eye Res; Botox-A improve the thyroid-associated ophthalmopathy (TAO) orbital fibroblast activation through inhibiting the TGF-β/Smad signaling.; Authors - Xin Qi
human	IF	Reprod Biomed Online; Stomatin-like protein 2 is involved in endometrial stromal cell proliferation and differentiation during decidualization in mice and humans.; Authors - Qian Feng
mouse	WB	BMC Nephrol; Adiponectin protects obesity-related glomerulopathy by inhibiting ROS/NF-κB/NLRP3 inflammation pathway.; Authors - Xiaohong Xu