c-Fos Polyklonaler Antikörper

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• KD/KO Validated

c-Fos Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

28

Rezensionen (2)

Kat-Nr. : 26192-1-AP

Synonyme

AP 1, C FOS, Cellular oncogene fos, c-Fos, FOS, G0S7, Proto oncogene c Fos

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Galerie der Validierungsdaten

• 

  WB analysis of RAW 264.7 using 26192-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 26192-1-AP (c-Fos antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 26192-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26192-1-AP (c-Fos antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 26192-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26192-1-AP (c-FOS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 26192-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 26192-1-AP (c-FOS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 26192-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 26192-1-AP (c-FOS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: RAW 264.7-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 12 publications below
• IHC: See 10 publications below
• IF: See 8 publications below

Produktinformation

26192-1-AP bindet in WB, IHC, IF, ELISA c-Fos und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hund, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: c-Fos fusion protein Ag24340
• Vollständiger Name: FOS
• Berechnetes Molekulargewicht: 41 kDa
• Beobachtetes Molekulargewicht: 65 kDa
• GenBank-Zugangsnummer: BC004490
• Gene symbol: FOS
• Gene ID (NCBI): 2353
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

c-Fos, also named as FOS and G0/G1 switch regulatory protein 7, is a 380 amino acid protein, which contains 1 bZIP (basic-leucine zipper) domain and belongs to the bZIP family. c-Fos is expressed at very low levels in quiescent cells. When cells are stimulated to reenter growth, c-Fos undergo 2 waves of expression, the first one peaks 7.5 minutes following FBS induction. At this stage, the c-Fos protein is localized endoplasmic reticulum. The second wave of expression occurs at about 20 minutes after induction and peaks at 1 hour. At this stage, the c-FOS protein becomes nuclear. c-Fos is a very short-lived intracellular protein, which is very easy to degrade. The calculated molecular weight of c-Fos is 40 kDa, but Phosphorylated c-Fos protein is about 60-65 kDa. It is involved in important cellular events, including cell proliferation, differentiation and survival; genes associated with hypoxia; and angiogenesis; which makes its dysregulation an important factor for cancer development. It can also induce a loss of cell polarity and epithelial-mesenchymal transition, leading to invasive and metastatic growth in mammary epithelial cells. Expression of c-Fos is an indirect marker of neuronal activity because c-Fos is often expressed when neurons fire action potentials. Upregulation of c-Fos mRNA in a neuron indicates recent activity.

Publikationen

Species	Application	Title
mouse	IHC, IF	Nature; Mechanisms of stretch-mediated skin expansion at single-cell resolution.; Authors - Mariaceleste Aragona
mouse	WB	Neuron; Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.; Authors - Xiaohui Li
mouse	IHC	Nat Commun; Ketamine activates adult-born immature granule neurons to rapidly alleviate depression-like behaviors in mice.; Authors - Radhika Rawat
mouse	IHC	Biol Psychiatry; Fighting Females: Neural and Behavioral Consequences of Social Defeat Stress in Female Mice.; Authors - Emily L Newman
human	IHC	Nat Commun; Excitatory VTA to DH projections provide a valence signal to memory circuits.; Authors - Yuan Han
mouse	IHC	Nat Commun; Activating newborn neurons suppresses depression and anxiety-like behaviors.; Authors - Elif Tunc-Ozcan

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Matthias (Verified Customer) (09-10-2019)

Good Antibody, very specific.

• Applications: Immunohistochemistry, Immunofluorescence,
• Primary Antibody Dilution: 2000
• Cell Tissue Type: Brain tissue

c-Fos Antibody Immunohistochemistry,Immunofluorescence, validation (2000 dilution) in Brain tissue (Cat no:26192-1-AP)

FH

Elif (Verified Customer) (06-21-2019)

It works better with 2 overnight incubation in 4 degrees.

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Dentate gyrus

c-Fos Antibody Immunohistochemistry, validation (1:500 dilution) in Dentate gyrus (Cat no:26192-1-AP)