IL-7 Rekombinanter Antikörper

IL-7 Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

Unkonjugiert

CloneNo.

241000F3

Kat-Nr. : 98206-1-RR

Synonyme

IL7, Interleukin-7, interleukin 7, IL 7, 241000F3

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  FC experiment of Transfected HEK-293T using 98206-1-RR

  1x10^6 HEK-293T cells or IL-7 transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Human IL-7 Rabbit Recombinant Antibody (98206-1-RR, Clone:241000F3) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of Transfected HEK-293T using 98206-1-RR

  1x10^6 HEK-293T cells (left) or IL-7 transfected HEK-293T cells (right) were intracellularly stained with 0.25 ug Anti-Human IL-7 Rabbit Recombinant Antibody (98206-1-RR, Clone:241000F3) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 98206-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98206-1-RR against Human IL-7 were performed. The affinity constant is 8.55 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): Transfected HEK-293T cells

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98206-1-RR bindet in FC (Intra) IL-7 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: interleukin 7
• Berechnetes Molekulargewicht: 20 kDa
• GenBank-Zugangsnummer: NM_000880.4
• Gene symbol: IL-7
• Gene ID (NCBI): 3574
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

Interleukin-7 (IL-7) is a cytokine involved in B and T cell development. It plays an active role in the development, survival, maintaining and restoring homeostasis of mature T lymphocytes and is a key regulator of the commitment, survival, proliferation and maturation of B cells during development. Furthermore, IL-7 can improve the antiviral function and expansion of natural killer (NK) cells and regulate the development and differentiation of dendritic cells. IL-7 has also been reported as a regulator of the development of central nervous system and myogenesis and skeletal muscle cell migration. (PMID: 29663382; 29655570; 29449560)