MYC-tag Monoklonaler Antikörper
MYC-tag Monoklonal Antikörper für IF, IP, WB, ELISA
Wirt / Isotyp
Maus / IgG1
Getestete Reaktivität
rekombinanten Protein und mehr (3)
Anwendung
WB, IP, IF, CoIP, ELISA
Konjugation
Unkonjugiert
CloneNo.
2D11A8
Kat-Nr. : 66004-1-Ig
Synonyme
"MYC-tag Antibodies" Comparison
View side-by-side comparison of MYC-tag antibodies from other vendors to find the one that best suits your research needs.
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | Recombinant protein |
| Erfolgreiche IP | Transfizierte HEK-293-Zellen |
| Erfolgreiche Detektion in IF | Transfizierte HEK-293-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:20000 |
| Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunfluoreszenz (IF) | IF : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| WB | See 28 publications below |
| IF | See 3 publications below |
| IP | See 7 publications below |
| CoIP | See 4 publications below |
Produktinformation
66004-1-Ig bindet in WB, IP, IF, CoIP, ELISA MYC-tag und zeigt Reaktivität mit rekombinanten Protein
| Getestete Reaktivität | rekombinanten Protein |
| In Publikationen genannte Reaktivität | human, Maus, Ratte |
| Wirt / Isotyp | Maus / IgG1 |
| Klonalität | Monoklonal |
| Typ | Antikörper |
| Immunogen | Peptid |
| Vollständiger Name | MYC-tag |
| Gene symbol | |
| Gene ID (NCBI) | |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Protein-A-Reinigung |
| Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The Myc tag corresponds to amino acid residues(EQKLISEEDL) of the human c-Myc protein. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. Myc-Tag mouse mAb detects recombinant proteins containing the Myc tag. The antibody recognizes the Myc-tag EQKLISEEDL fused to either the amino- or carboxy- terminus of targeted proteins.
Protokolle
| Produktspezifische Protokolle | |
|---|---|
| WB protocol for MYC-tag antibody 66004-1-Ig | Protokoll herunterladen |
| IF protocol for MYC-tag antibody 66004-1-Ig | Protokoll herunterladen |
| IP protocol for MYC-tag antibody 66004-1-Ig | Protokoll herunterladen |
| Standard-Protokolle | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Mol Cell PTPS Facilitates Compartmentalized LTBP1 S-Nitrosylation and Promotes Tumor Growth under Hypoxia. | ||
Circ Res Yes-Associated Protein Promotes Angiogenesis Via Signal Transducer and Activator of Transcription 3 in Endothelial Cells. | ||
Nat Commun The ubiquitin ligase UBR5 suppresses proteostasis collapse in pluripotent stem cells from Huntington's disease patients. | ||
Cell Res ERK kinase phosphorylates and destabilizes the tumor suppressor FBW7 in pancreatic cancer. |
