NAPRT1 Polyklonaler Antikörper

NAPRT1 Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

14

Kat-Nr. : 13549-1-AP

Synonyme

FHA HIT interacting protein, FHIP, NAPRT1, NAPRTase, PP3856

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Galerie der Validierungsdaten

• 

  WB analysis using 13549-1-AP

  Mouse liver tissue were subjected to SDS PAGE followed by western blot with 13549-1-AP (NAPRT1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 13549-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 13549-1-AP (NAPRT1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human colon using 13549-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 13549-1-AP (NAPRT1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 13549-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13549-1-AP (NAPRT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 13549-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 13549-1-AP (NAPRT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 13549-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13549-1-AP (NAPRT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 13549-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13549-1-AP (NAPRT1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 13549-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13549-1-AP (NAPRT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 13549-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13549-1-AP (NAPRT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human small intestine using 13549-1-AP

  human small intestine tissue were subjected to SDS PAGE followed by western blot with 13549-1-AP (NAPRT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 13549-1-AP

  IP Result of anti-NAPRT1 (IP:13549-1-AP, 4ug; Detection:13549-1-AP 1:1000) with HEK-293 cells lysate 2800ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, HEK-293-Zellen, HepG2-Zellen, humanes Kolongewebe, L02-Zellen, Mausnierengewebe
• Erfolgreiche IP: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 13 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Produktinformation

13549-1-AP bindet in WB, IP, IHC, IF, ELISA NAPRT1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NAPRT1 fusion protein Ag4265
• Vollständiger Name: nicotinate phosphoribosyltransferase domain containing 1
• Berechnetes Molekulargewicht: 514 aa, 55 kDa
• Beobachtetes Molekulargewicht: 51 kDa, 56 kDa
• GenBank-Zugangsnummer: BC032466
• Gene symbol: NAPRT1
• Gene ID (NCBI): 93100
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nicotinic acid (NA) is a coenzyme in cellular redox reactions, and is an essential component of metabolic pathways in all living cells. NAPRT1 (Nicotinate phosphoribosyltransferase) is essential for increasing cellular NAD levels and, thus, to prevent oxidative stress of cells. NAPRT1 converts Nicotinic acid (NA; niacin) to NA mononucleotide (NaMN), which is then converted to NA adenine dinucleotide (NaAD), and finally to nicotinamide adenine dinucleotide (NAD).

Publikationen

Species	Application	Title
human,mouse	WB	Cell Metab; NAD+ Metabolism Maintains Inducible PD-L1 Expression to Drive Tumor Immune Evasion.; Authors - Hongwei Lv
human	IF,WB	J Pharm Anal; Epigenetic drug library screening reveals targeting DOT1L abrogates NAD+ synthesis by reprogramming H3K79 methylation in uveal melanoma; Authors - Xiang Gu
	WB	Kidney Int; Anticystogenic activity of a small molecule PAK4 inhibitor may be a novel treatment for autosomal dominant polycystic kidney disease.; Authors - Vicki J Hwang
human	WB	Clin Cancer Res; Targeting of NAD metabolism in pancreatic cancer cells: potential novel therapy for pancreatic tumors.; Authors - Chini Claudia Cs CC
human,mouse	WB,IHC	Int J Mol Sci; NAD+ Anabolism Disturbance Causes Glomerular Mesangial Cell Injury in Diabetic Nephropathy.; Authors - Xue Li
human,mouse	IF	Metabolism; Hyperglycemia-reduced NAD+ biosynthesis impairs corneal epithelial wound healing in diabetic mice.; Authors - Ya Li