PCNA Monoklonaler Antikörper
PCNA Monoklonal Antikörper für WB,ELISA
Wirt / Isotyp
Maus / IgG
Getestete Reaktivität
human, Maus
Anwendung
WB,ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 65034-1-Ig
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | 293 cells |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Produktinformation
65034-1-Ig bindet in WB,ELISA PCNA und zeigt Reaktivität mit human, Maus
| Getestete Reaktivität | human, Maus |
| Wirt / Isotyp | Maus / IgG |
| Klonalität | Monoklonal |
| Typ | Antikörper |
| Immunogen | Rekombinantes Protein |
| Vollständiger Name | proliferating cell nuclear antigen |
| Berechnetes Molekulargewicht | 29 kDa/31 kDa |
| Beobachtetes Molekulargewicht | 36 kDa |
| GenBank-Zugangsnummer | BC000491 |
| Gene symbol | PCNA |
| Gene ID (NCBI) | 5111 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Antigen-Affinitätsreinigung |
| Lagerungspuffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Lagerungsbedingungen | Bei 2-8℃ lagern. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
PCNA, also named as Cyclin, belongs to the PCNA family. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. PCNA induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. The PCNA protein has been highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. This antibody is a mouse monoclonal antibody raised against recombinant protein of human PCNA. The calculated molecular weight of PCNA is 29 kDa, but modified PCNA is 36kDa （PMID: 1358458）.
