CD31 Monoklonaler Antikörper

CD31 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

26

CloneNo.

2A1E2

Kat-Nr. : 66065-1-Ig

Synonyme

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Galerie der Validierungsdaten

• 

  WB analysis of Jurkat using 66065-1-Ig

  Untreated and PNGase F-treated lysates of Jurkat cells were subjected to SDS PAGE followed by western blot with 66065-1-Ig (CD31 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

• 

  WB analysis using 66065-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66065-1-Ig (CD31 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of THP-1 using 66065-1-Ig

  Untreated and PNGase F-treated lysates of THP-1 cells were subjected to SDS PAGE followed by western blot with 66065-1-Ig (CD31 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

• 

  WB analysis of Jurkat using 66065-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66065-1-Ig (PECAM1,CD31 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 66065-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66065-1-Ig (CD31 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66065-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66065-1-Ig (CD31 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66065-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66065-1-Ig (CD31 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66065-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66065-1-Ig (CD31 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66065-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66065-1-Ig (CD31 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66065-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66065-1-Ig (CD31 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human tonsillitis using 66065-1-Ig

  Immunofluorescent analysis of (4% PFA ) fixed human tonsillitis tissue using 66065-1-Ig(CD31 antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human tonsillitis using 66065-1-Ig

  Immunofluorescent analysis of (4% PFA ) fixed human tonsillitis tissue using 66065-1-Ig(CD31 antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, humanes Plazenta-Gewebe, THP-1-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Mammakarzinomgewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Tonsillitisgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:8000
• Immunhistochemie (IHC): IHC : 1:400-1:4000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 5 publications below
• IF: See 11 publications below

Produktinformation

66065-1-Ig bindet in WB, IHC, IF, ELISA CD31 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD31 fusion protein Ag1787
• Vollständiger Name: platelet/endothelial cell adhesion molecule
• Berechnetes Molekulargewicht: 83 kDa
• Beobachtetes Molekulargewicht: 120-130 kDa
• GenBank-Zugangsnummer: BC022512
• Gene symbol: PECAM1
• Gene ID (NCBI): 5175
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

Publikationen

Species	Application	Title
human	IF	Nature; Construction of a human cell landscape at single-cell level.; Authors - Xiaoping Han
		Biomaterials; An iRGD-conjugated prodrug micelle with blood-brain-barrier penetrability for anti-glioma therapy.; Authors - Lu Lu
human	IF	Small; Airflow-Assisted 3D Bioprinting of Human Heterogeneous Microspheroidal Organoids with Microfluidic Nozzle.; Authors - Haiming Zhao
human	IF	Theranostics; Single-cell landscape reveals active cell subtypes and their interaction in the tumor microenvironment of gastric cancer.; Authors - Yumin Li
	IF	Theranostics; Encapsulation of LXR ligand by D-Nap-GFFY hydrogel enhances anti-tumorigenic actions of LXR and removes LXR-induced lipogenesis.; Authors - Ke Feng
	IHC	Cancer Lett; CD44(+) tumor cells promote early angiogenesis in head and neck squamous cell carcinoma.; Authors - Nils Ludwig

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