PER2 Polyklonaler Antikörper

PER2 Polyklonal Antikörper für IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

16

Kat-Nr. : 20359-1-AP

Synonyme

FASPS, hPER2, KIAA0347, PER2, period homolog 2 (Drosophila)

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Galerie der Validierungsdaten

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  WB analysis of BxPC-3 using 20359-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 20359-1-AP (PER2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 20359-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 20359-1-AP (PER2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 20359-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 20359-1-AP (PER2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 20359-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 20359-1-AP (PER2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 20359-1-AP

  IP Result of anti-PER2 (IP:20359-1-AP, 3ug; Detection:20359-1-AP 1:300) with HEK-293 cells lysate 1000ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BxPC-3-Zellen, K-562-Zellen, L02-Zellen, Y79-Zellen
• Erfolgreiche IP: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 14 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Produktinformation

20359-1-AP bindet in WB, IP, IHC, IF, ELISA PER2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: period homolog 2 (Drosophila)
• Berechnetes Molekulargewicht: 137 kDa
• Beobachtetes Molekulargewicht: 136 kDa
• GenBank-Zugangsnummer: NM_022817
• Gene symbol: PER2
• Gene ID (NCBI): 8864
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PER2, also named as KIAA0347, is a component of the circadian clock mechanism which is essential for generating circadian rhythms. PER2 is a negative element in the circadian transcriptional loop. PER2 influences clock function by interacting with other circadian regulatory proteins and transporting them to the nucleus. It negatively regulates CLOCK|NPAS2-BMAL1|BMAL2-induced transactivation. Defects in PER2 are a cause of familial advanced sleep-phase syndrome (FASPS) which is characterized by very early sleep onset and offset. The antibody is specific to PER2. It can recognize isoform1 and isoform2 of PER2.

Publikationen

Species	Application	Title
human	WB	Cell; Acid Suspends the Circadian Clock in Hypoxia through Inhibition of mTOR.; Authors - Zandra E Walton
human	WB	Cell Metab; MYC Disrupts the Circadian Clock and Metabolism in Cancer Cells.; Authors - Brian J Altman
human	WB	Sci Adv; Time-of-day specificity of anticancer drugs may be mediated by circadian regulation of the cell cycle.; Authors - Yool Lee
human	WB	Nat Commun; Correspondence: Oncogenic MYC persistently upregulates the molecular clock component REV-ERBα.; Authors - Brian J Altman
human	WB, IF, IP	Elife; KPNB1 mediates PER/CRY nuclear translocation and circadian clock function.; Authors - Yool Lee
mouse	WB	Elife; NRF2 regulates core and stabilizing circadian clock loops, coupling redox and timekeeping inMus musculus.; Authors - Ryan S Wible