SMO Monoklonaler Antikörper

SMO Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Hund, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

19

CloneNo.

2B10A11

Rezensionen (2)

Kat-Nr. : 66851-1-Ig

Synonyme

Gx, Protein Gx, SMO, SMOH, Smoothened homolog

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Galerie der Validierungsdaten

• 

  WB analysis using 66851-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66851-1-Ig (SMO antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 66851-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66851-1-Ig (SMO antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 66851-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66851-1-Ig (SMO antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MDCK using 66851-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using 66851-1-Ig (SMO antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenhirngewebe, Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MDCK-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 19 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

66851-1-Ig bindet in WB, IHC, IF, ELISA SMO und zeigt Reaktivität mit human, Hund, Maus, Ratten

• Getestete Reaktivität: human, Hund, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SMO fusion protein Ag25861
• Vollständiger Name: smoothened homolog (Drosophila)
• Berechnetes Molekulargewicht: 787 aa, 86 kDa
• Beobachtetes Molekulargewicht: 86 kDa
• GenBank-Zugangsnummer: BC009989
• Gene symbol: SMO
• Gene ID (NCBI): 6608
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Smoothened (SMO) is a G protein-coupled receptor that is a component of the hedgehog (Hh) signaling pathway and is conserved from flies to humans. The Hh pathway plays central roles in animal development and stem-cell function. Defects in Hh signalling lead to birth defects and cancer in humans. Expressed on the primary cilium, SMO interacts with the patched protein, a receptor for Hh proteins.

Publikationen

Species	Application	Title
human	WB,IHC,IF	Int J Biol Sci; Drug Repurposing Flubendazole to Suppress Tumorigenicity via PCSK9-dependent Inhibition and Potentiate Lenvatinib Therapy for Hepatocellular Carcinoma; Authors - Wenjiao Jin
human,mouse	WB	iScience; Mutational burden of XPNPEP3 leads to defects in mitochondrial complex I and cilia in NPHPL1; Authors - Lingxiao Tong
human	WB	Front Cell Dev Biol; Microtubule Stabilization Enhances the Chondrogenesis of Synovial Mesenchymal Stem Cells.; Authors - Jiawei Li
rat	WB	Oxid Med Cell Longev; Mitochondrial Protection and Against Glutamate Neurotoxicity via Shh/Ptch1 Signaling Pathway to Ameliorate Cognitive Dysfunction by Kaixin San in Multi-Infarct Dementia Rats; Authors - Xiaoqiong Li
human	WB	Cancer Sci; Transcription factor POU4F2 promotes colorectal cancer cell migration and invasion through hedgehog-mediated epithelial-mesenchymal transition.; Authors - Kaibo Guo
human	WB	FASEB J; Inhibition of CK2α accelerates skin wound healing by promoting endothelial cell proliferation through the Hedgehog signaling pathway; Authors - Junjie Zhu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Aidan (Verified Customer) (08-31-2021)

Smo Monoclonal antibody responded to S. purpuratus Smo protein in both IF and WB experiments. For IF, methanol fixed embryos treated with 1:100 dilution of the antibody in PBS overnight, followed by incubation in a 1:300 dilution of secondary antibody Alexa Goat anti-mouse 488 diluted 1:300 in PBS. Localization appears peri-nuclear at the 16 cell stage.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: IF: 1:100 WB: 1:2000
• Cell Tissue Type: Strongylocentrotus purpuratus (Purple Sea Urchin) Embryo

SMO Antibody Western Blot,Immunofluorescence validation (IF: 1:100 WB: 1:2000 dilution) in Strongylocentrotus purpuratus (Purple Sea Urchin) Embryo (Cat no:66851-1-Ig)

FH

Boyan (Verified Customer) (04-24-2019)

By WB, this antibody primarily labeled a band around 110 kD; by IF, it cannot label the ciliary localisation of Smo when the cells were stimulated for Hh pathway.

• Applications: Western Blot, Immunofluorescence,
• Cell Tissue Type: MEF