Synaptophysin Monoklonaler Antikörper

Synaptophysin Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG3

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

13

CloneNo.

5B3F2

Kat-Nr. : 60191-1-Ig

Synonyme

synaptophysin, SYP

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Galerie der Validierungsdaten

• 

  WB analysis of fetal human brain using 60191-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60191-1-Ig (Synaptophysin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 60191-1-Ig

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 60191-1-Ig (Synaptophysin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 60191-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 60191-1-Ig (Synaptophysin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 60191-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 60191-1-Ig (SYP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 60191-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60191-1-Ig (Synaptophysin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 60191-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60191-1-Ig (Synaptophysin antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of PC-12 using 60191-1-Ig

  Immunofluorescent analysis of PC-12 cells using 60191-1-Ig (Synaptophysin antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: fetales humanes Hirngewebe, Hausschwein-Hirngewebe, humanes Hirngewebe, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: PC-12-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 9 publications below
• FC: See 1 publications below

Produktinformation

60191-1-Ig bindet in WB, IHC, IF, FC, ELISA Synaptophysin und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG3
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Synaptophysin fusion protein Ag11803
• Vollständiger Name: synaptophysin
• Berechnetes Molekulargewicht: 313 aa, 34 kDa
• Beobachtetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: BC064550
• Gene symbol: SYP
• Gene ID (NCBI): 6855
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Synaptophysin (SYP, also known as major synaptic vesicle protein p38) is a 38-kDa integral membrane glycoprotein that regulates synaptic vesicle endocytosis. It is the most abundant synaptic vesicle protein by mass. Synaptophysin is present in neuroendocrine cells and neurons that participate in synaptic transmission. Synaptophysin is a useful marker for identification of neuroendocrine cells and neoplasms. (PMID: 3010302; 21658579)

Publikationen

Species	Application	Title
mouse	WB,IF	Brain Behav Immun; l-Cysteine suppresses hypoxia-ischemia injury in neonatal mice by reducing glial activation, promoting autophagic flux and mediating synaptic modification via H2S formation.; Authors - Danqing Xin
human	IF	Nat Protoc; Nanoscale imaging of clinical specimens using conventional and rapid-expansion pathology.; Authors - Octavian Bucur
mouse	FC	Aging Cell; Gamma frequency light flicker regulates amyloid precursor protein trafficking for reducing β-amyloid load in Alzheimer's disease model.; Authors - Qi Shen
mouse	WB,IF	Cell Death Dis; Phosphorylation of Parkin at serine 131 by p38 MAPK promotes mitochondrial dysfunction and neuronal death in mutant A53T α-synuclein model of Parkinson's disease.; Authors - Jialong Chen
mouse	IF	FASEB J; Silencing of circIgf1r plays a protective role in neuronal injury via regulating astrocyte polarization during epilepsy.; Authors - Lin Shao
mouse	IF,WB	Chemosphere; Arsenic exposure via drinking water during pregnancy and lactation induces autism-like behaviors in male offspring mice.; Authors - Xing Zhang

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