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CoraLite® Plus 488-conjugated Synaptophysin Monoclonal antibody

Synaptophysin Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig, Mouse, Rat

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4E12C4

Cat no : CL488-67864

Synonyms

synaptophysin, SYP

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite® Plus 488-conjugated Synaptophysin Monocl (CL488-67864)

    IF Staining of mouse brain using CL488-67864

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 Synaptophysin antibody (CL488-67864, Clone: 4E12C4 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite® Plus 488-conjugated Synaptophysin Monocl (CL488-67864)

    IF Staining of mouse brain using CL488-67864

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 Synaptophysin antibody (CL488-67864, Clone: 4E12C4 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of PC-12 cells using CoraLite® Plus 488-conjugated Synaptophysin Monocl (CL488-67864)

    FC experiment of PC-12 using CL488-67864

    1X10^6 PC-12 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Synaptophysin (CL488-67864, Clone:4E12C4) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inmouse brain tissue
Positive FC detected inPC-12 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67864 targets Synaptophysin in IF, FC (Intra) applications and shows reactivity with Human, Pig, Mouse, Rat samples.

Tested Reactivity Human, Pig, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Synaptophysin fusion protein Ag11803
Full Name synaptophysin
Calculated Molecular Weight 313 aa, 34 kDa
GenBank Accession NumberBC064550
Gene Symbol SYP
Gene ID (NCBI) 6855
RRIDAB_2909472
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Synaptophysin (SYP, also known as major synaptic vesicle protein p38) is a 38-kDa integral membrane glycoprotein that regulates synaptic vesicle endocytosis. It is the most abundant synaptic vesicle protein by mass. Synaptophysin is present in neuroendocrine cells and neurons that participate in synaptic transmission. Synaptophysin is a useful marker for identification of neuroendocrine cells and neoplasms. (PMID: 3010302; 21658579)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Synaptophysin antibody CL488-67864Download protocol
FC protocol for CL Plus 488 Synaptophysin antibody CL488-67864Download protocol
Standard Protocols
Click here to view our Standard Protocols