Anticorps Monoclonal anti-beta actin

beta actin Monoclonal Antibody for ELISA

Hôte / Isotype

Mouse / IgM

Réactivité testée

Humain et plus (1)

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(9)

CloneNo.

7A9H3

N° de cat : 60008-2-Ig

Synonymes

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Galerie de données de validation

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  WB analysis of HeLa using 60008-2-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60008-2-Ig (ACTB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 60008-2-Ig

  MCF7 cells were subjected to SDS PAGE followed by western blot with 60008-2-Ig (ACTB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 60008-2-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60008-2-Ig (ACTB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 60008-2-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 60008-2-Ig (ACTB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 60008-2-Ig

  Immunohistochemical analysis of paraffin-embedded human heart using 60008-2-Ig(ACTB antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 60008-2-Ig

  Immunohistochemical analysis of paraffin-embedded human heart using 60008-2-Ig(ACTB antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 60008-2-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney using 60008-2-Ig(ACTB antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 60008-2-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney using 60008-2-Ig(ACTB antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 60008-2-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta using 60008-2-Ig(ACTB antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 60008-2-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta using 60008-2-Ig(ACTB antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 60008-2-Ig

  Immunohistochemical analysis of paraffin-embedded human testis using 60008-2-Ig(ACTB antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 60008-2-Ig

  Immunohistochemical analysis of paraffin-embedded human testis using 60008-2-Ig(ACTB antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 60008-2-Ig

  Immunohistochemical analysis of paraffin-embedded human skin using 60008-2-Ig(ACTB antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 60008-2-Ig

  Immunohistochemical analysis of paraffin-embedded human skin using 60008-2-Ig(ACTB antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 60008-2-Ig

  Immunohistochemical analysis of paraffin-embedded human brain using 60008-2-Ig(ACTB antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 60008-2-Ig

  Immunohistochemical analysis of paraffin-embedded human brain using 60008-2-Ig(ACTB antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 60008-2-Ig

  Immunohistochemical analysis of paraffin-embedded human spleen using 60008-2-Ig(ACTB antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 60008-2-Ig

  Immunohistochemical analysis of paraffin-embedded human spleen using 60008-2-Ig(ACTB antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 60008-2-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary using 60008-2-Ig(ACTB antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 60008-2-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary using 60008-2-Ig(ACTB antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human colon using 60008-2-Ig

  Immunohistochemical analysis of paraffin-embedded human colon using 60008-2-Ig(ACTB antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 60008-2-Ig

  Immunohistochemical analysis of paraffin-embedded human colon using 60008-2-Ig(ACTB antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 60008-2-Ig

  Immunofluorescent analysis of fixed Hela cells using 60008-2-Ig (beta actin antibody) at dilution of 1:25 and Rhodamine-Goat anti-mouse IgG.

• 

  IF Staining of MCF-7 using 60008-2-Ig

  Immunofluorescent analysis of fixed MCF-7 cells using 60008-2-Ig (beta actin antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG.

• 

  IF Staining of HepG2 using 60008-2-Ig

  Immunofluorescent analysis of HepG2 cells, using 60008-2-lg and Rhodamine-labeled goat anti-mouse IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications publiées

• WB: See 7 publications below

Informations sur le produit

60008-2-Ig cible beta actin dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgM
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: beta actin Protéine recombinante Ag0297
• Nom complet: actin, beta
• Masse moléculaire calculée: 375 aa, 42 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC002409
• Symbole du gène: Beta Actin
• Identification du gène (NCBI): 60
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Précipitation de l'acide caprylique/du sulfate d'ammonium
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. This antibody can recognize all of actins.

Publications

Species	Application	Title
human,mouse	WB	J Pharm Anal; Fangchinoline induces antiviral response by suppressing STING degradation; Authors - Jinyong Wang
human		Front Oncol; Fbw7 Inhibits the Progression of Activated B-Cell Like Diffuse Large B-Cell Lymphoma by Targeting the Positive Feedback Loop of the LDHA/lactate/miR-223 Axis.; Authors - Su Yao
		J Exp Clin Cancer Res; Fbw7 regulates apoptosis in activated B-cell like diffuse large B-cell lymphoma by targeting Stat3 for ubiquitylation and degradation.; Authors - Su Yao
human	WB	Amino Acids; Quantitative proteomic dissection of a native 14-3-3ε interacting protein complex associated with hepatocellular carcinoma.; Authors - Chen Bai
mouse	WB	Mol Cell Endocrinol; Maternal obesity interrupts the coordination of the unfolded protein response and heat shock response in the postnatal developing hypothalamus of male offspring in mice.; Authors - Nan Chen
mouse	WB	Front Endocrinol (Lausanne); Pre-Weaning Exposure to Maternal High-Fat Diet Is a Critical Developmental Window for Programming the Metabolic System of Offspring in Mice.; Authors - Hong Yang

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