Anticorps Monoclonal anti-beta actin

beta actin Monoclonal Antibody for ELISA

Hôte / Isotype

Mouse / IgM

Réactivité testée

Humain et plus (3)

Applications

WB, IP, IF, ELISA

Conjugaison

Non conjugué

Publications

32

CloneNo.

7A9H3

N° de cat : 60008-2-Ig

Synonymes

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Galerie de données de validation

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  WB analysis of HeLa using 60008-2-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60008-2-Ig (ACTB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 60008-2-Ig

  MCF7 cells were subjected to SDS PAGE followed by western blot with 60008-2-Ig (ACTB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 60008-2-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60008-2-Ig (ACTB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 60008-2-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 60008-2-Ig (ACTB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 60008-2-Ig

  Immunohistochemical analysis of paraffin-embedded human heart using 60008-2-Ig(ACTB antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 60008-2-Ig

  Immunohistochemical analysis of paraffin-embedded human heart using 60008-2-Ig(ACTB antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 60008-2-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney using 60008-2-Ig(ACTB antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 60008-2-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney using 60008-2-Ig(ACTB antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 60008-2-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta using 60008-2-Ig(ACTB antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 60008-2-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta using 60008-2-Ig(ACTB antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 60008-2-Ig

  Immunohistochemical analysis of paraffin-embedded human testis using 60008-2-Ig(ACTB antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 60008-2-Ig

  Immunohistochemical analysis of paraffin-embedded human testis using 60008-2-Ig(ACTB antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 60008-2-Ig

  Immunohistochemical analysis of paraffin-embedded human skin using 60008-2-Ig(ACTB antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 60008-2-Ig

  Immunohistochemical analysis of paraffin-embedded human skin using 60008-2-Ig(ACTB antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 60008-2-Ig

  Immunohistochemical analysis of paraffin-embedded human brain using 60008-2-Ig(ACTB antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 60008-2-Ig

  Immunohistochemical analysis of paraffin-embedded human brain using 60008-2-Ig(ACTB antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 60008-2-Ig

  Immunohistochemical analysis of paraffin-embedded human spleen using 60008-2-Ig(ACTB antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 60008-2-Ig

  Immunohistochemical analysis of paraffin-embedded human spleen using 60008-2-Ig(ACTB antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 60008-2-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary using 60008-2-Ig(ACTB antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human colon using 60008-2-Ig

  Immunohistochemical analysis of paraffin-embedded human colon using 60008-2-Ig(ACTB antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 60008-2-Ig

  Immunohistochemical analysis of paraffin-embedded human colon using 60008-2-Ig(ACTB antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 60008-2-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary using 60008-2-Ig(ACTB antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 60008-2-Ig

  Immunofluorescent analysis of HepG2 cells, using 60008-2-lg and Rhodamine-labeled goat anti-mouse IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  IF Staining of HeLa using 60008-2-Ig

  Immunofluorescent analysis of fixed Hela cells using 60008-2-Ig (beta actin antibody) at dilution of 1:25 and Rhodamine-Goat anti-mouse IgG.

• 

  IF Staining of MCF-7 using 60008-2-Ig

  Immunofluorescent analysis of fixed MCF-7 cells using 60008-2-Ig (beta actin antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG.

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Applications testées

Dilution recommandée

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 27 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Informations sur le produit

60008-2-Ig cible beta actin dans les applications de WB, IP, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, bovin, Humain, souris
• Hôte / Isotype: Mouse / IgM
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: beta actin Protéine recombinante Ag0297
• Nom complet: actin, beta
• Masse moléculaire calculée: 375 aa, 42 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC002409
• Symbole du gène: ACTB
• Identification du gène (NCBI): 60
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Précipitation de l'acide caprylique/du sulfate d'ammonium
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. This antibody can recognize all of actins.

Publications

Species	Application	Title
human	IP	Nat Commun; The methyltransferase METTL3 negatively regulates nonalcoholic steatohepatitis (NASH) progression.; Authors - Xinzhi Li
mouse	WB	Cell Mol Immunol; MEF2C promotes M1 macrophage polarization and Th1 responses.; Authors - Xibao Zhao
human	WB	Elife; Hepatic conversion of acetyl-CoA to acetate plays crucial roles in energy stress; Authors - Jinyang Wang
human	IP	Elife; Capping protein regulates endosomal trafficking by controlling F-actin density around endocytic vesicles and recruiting RAB5 effectors.; Authors - Dawei Wang
rat	WB	Bioeng Transl Med; Neonatal microglia and proteinase inhibitors-treated adult microglia improve traumatic brain injury in rats by resolving the neuroinflammation.; Authors - Ping Zheng
mouse	WB	PLoS Genet; Neuroplastin genetically interacts with Cadherin 23 and the encoded isoform Np55 is sufficient for cochlear hair cell function and hearing.; Authors - Sherylanne Newton