Anticorps Monoclonal anti-CD11b/c

CD11b/c Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG2a, kappa

Réactivité testée

rat

Applications

FC

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

OX42

N° de cat : CL488-65229

Synonymes

CD11b, integrin alpha M, Itgam, OX42

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Galerie de données de validation

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  FC experiment of rat splenocytes using CL488-65229

  1X10^6 rat splenocytes were surface stained with 0.5 ug CoraLite® Plus 488 Anti-Rat CD11b/c (CL488-65229, Clone:OX42) or 0.5 ug Isotype Control. Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: splénocytes de rat

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-65229 cible CD11b/c dans les applications de FC et montre une réactivité avec des échantillons rat

• Réactivité: rat
• Hôte / Isotype: Mouse / IgG2a, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Rat peritoneal macrophages
• Nom complet: integrin alpha M
• Masse moléculaire calculée: 127 kDa
• Numéro d’acquisition GenBank: NM_012711
• Symbole du gène: CD11b
• Identification du gène (NCBI): 25021
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b (integrin alpha M or CR3A) and CD11c (integrin alpha X) are members of the integrin alpha chain family and can form α/β heterodimers with CD18 (integrin β2). CD11b/CD18 is a receptor for the complement protein fragment C3bi, and is also a receptor for fibrinogen, factor X, and ICAM1 (PMID: 2971974; 15485828). CD11c/CD18 acts as a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis (PMID: 1671533). The OX-42 antibody recognizes a common epitope shared between CD11b and CD11c. It binds to C3bi receptor and is known to block C3bi binding (PMID: 3512425; 8449218).

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