Anticorps Monoclonal anti-CD11b/c

CD11b/c Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG2a, kappa

Réactivité testée

rat et plus (1)

Applications

IF, FC

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

Publications(1)

CloneNo.

OX42

N° de cat : CL647-65229

Synonymes

CD11b, integrin alpha M, Itgam, OX42

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Galerie de données de validation

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  FC experiment of wistar rat splenocytes using CL647-65229

  1X10^6 Wistar rat splenocytes were surface stained with 0.5 ug CoraLite® Plus 647 Anti-Rat CD11b/c (CL647-65229, Clone: OX42) or 5 ul Control Antibody. Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: Wistar rat splenocytes

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

CL647-65229 cible CD11b/c dans les applications de IF, FC et montre une réactivité avec des échantillons rat

• Réactivité: rat
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2a, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Rat peritoneal macrophages
• Nom complet: integrin alpha M
• Masse moléculaire calculée: 127 kDa
• Numéro d’acquisition GenBank: NM_012711
• Symbole du gène: CD11b
• Identification du gène (NCBI): 25021
• Conjugaison: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b (integrin alpha M or CR3A) and CD11c (integrin alpha X) are members of the integrin alpha chain family and can form α/β heterodimers with CD18 (integrin β2). CD11b/CD18 is a receptor for the complement protein fragment C3bi, and is also a receptor for fibrinogen, factor X, and ICAM1 (PMID: 2971974; 15485828). CD11c/CD18 acts as a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis (PMID: 1671533). The OX-42 antibody recognizes a common epitope shared between CD11b and CD11c. It binds to C3bi receptor and is known to block C3bi binding (PMID: 3512425; 8449218).

Publications

Species	Application	Title
human	IF	Cell Biosci; Tie2-expressing monocytes/macrophages promote angiogenesis in chronically ischaemic brain tissue; Authors - Chuyang Tai

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