Anticorps Recombinant de lapin anti-CXCL4/PF4

CXCL4/PF4 Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC (Intra)

Conjugaison

Non conjugué

CloneNo.

243087D5

N° de cat : 98410-2-RR

Synonymes

CXCL4, PF4, SCYB4, C X C motif chemokine 4, C-X-C motif chemokine 4

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Galerie de données de validation

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  FC experiment of human PBMCs using 98410-2-RR

  1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human CXCL4/PF4 Rabbit RecAb (98410-2-RR, Clone: 243087D5) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Human CD14. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of human PBMCs using 98410-2-RR

  1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human CXCL4/PF4 Rabbit RecAb (98410-2-RR, Clone: 243087D5) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Monocytes were gated.

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Applications testées

• Résultats positifs en FC (Intra): CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98410-2-RR cible CXCL4/PF4 dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CXCL4/PF4 Protéine recombinante Eg2129
• Nom complet: platelet factor 4
• Masse moléculaire calculée: 11 kDa
• Numéro d’acquisition GenBank: NM_002619.4
• Symbole du gène: PF4
• Identification du gène (NCBI): 5196
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

CXCL4, also known as platelet factor 4 (PF-4), is the oldest member of the chemokine family. CXCL4 is a chemokine produced by activated platelets and immune cells involved in pathological conditions such as cancer, infections and inflammatory diseases like systemic sclerosis (SSc), rheumatoid arthritis (RA) and psoriatic arthritis (PsA), among others. CXCL4 is present in the α-granules of platelets in amounts that constitute up to 2% platelet protein mass. CXCL4 plays a determinant role in distinct physiological processes such as in hematopoiesis, angiogenesis, coagulation and modulation of immune responses. For instance, CXCL4 can prevent monocyte apoptosis and promote cell survival, induces the production of TNF and reactive oxygen species (ROS) and promotes monocyte differentiation into a unique macrophage-like phenotype.

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