Anticorps Monoclonal anti-Desmin

Desmin Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc et plus (2)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

8

CloneNo.

3E12C8

N° de cat : 60226-1-Ig

Synonymes

CMD1I, CSM1, CSM2, DES, desmin

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Galerie de données de validation

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  WB analysis using 60226-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60226-1-Ig (Desmin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 60226-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 60226-1-Ig (DES antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: Coeur humain, tissu cardiaque de porc, tissu cardiaque humain, tissu de côlon de porc, tissu placentaire humain, tissu testiculaire humain

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Informations sur le produit

60226-1-Ig cible Desmin dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Desmin Protéine recombinante Ag9675
• Nom complet: desmin
• Masse moléculaire calculée: 470 aa, 54 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC032116
• Symbole du gène: DES
• Identification du gène (NCBI): 1674
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Desmin is the main intermediate filament protein in skeletal and cardiac muscle cells and is essential for both the structural integrity and the survival of muscle cells. As an abundant muscle-specific protein, desmin has been widely used as a marker of muscle derived tumors. Anti-desmin is also valuable in the differential diagnosis of tumors of uncertain origin.

Publications

Species	Application	Title
mouse	WB	Br J Pharmacol; Catalpol Alleviates Adriamycin-Induced Nephropathy by Activating the SIRT1 Signalling Pathway In Vivo and In Vitro.; Authors - Jiangnan Zhang
rat	IF	Diabetes Metab Res Rev; Gene expression profiles of rat MMECs with different glucose levels and fgl2 gene silencing.; Authors - Zhenzhong Zheng
mouse	IHC	Exp Cell Res; Effect of sarcolipin-mediated cell transdifferentiation in sarcopenia-associated skeletal muscle fibrosis.; Authors - Qiu-Nan Chen
human	IF	Exp Eye Res; Botox-A improve the thyroid-associated ophthalmopathy (TAO) orbital fibroblast activation through inhibiting the TGF-β/Smad signaling.; Authors - Xin Qi
human	IF	Reprod Biomed Online; Stomatin-like protein 2 is involved in endometrial stromal cell proliferation and differentiation during decidualization in mice and humans.; Authors - Qian Feng
mouse	WB	BMC Nephrol; Adiponectin protects obesity-related glomerulopathy by inhibiting ROS/NF-κB/NLRP3 inflammation pathway.; Authors - Xiaohong Xu