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Anticorps Polyclonal de lapin anti-EEA1

EEA1 Polyclonal Antibody for ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

, ELISA

Conjugaison

Non conjugué

N° de cat : 22266-1-AP

Synonymes

EEA1

Galerie de données de validation

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  • Western Blot (WB) analysis of NIH/3T3 cells using EEA1 Polyclonal antibody (22266-1-AP)

    WB analysis of NIH/3T3 using 22266-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 22266-1-AP (EEA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Applications testées

Dilution recommandée

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

22266-1-AP cible EEA1 dans les applications de , ELISA et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Réactivité citéeHumain
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène Peptide
Nom complet early endosome antigen 1
Masse moléculaire calculée 162 kDa
Numéro d’acquisition GenBankNM_003566
Symbole du gène EEA1
Identification du gène (NCBI) 8411
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Early endosome antigen 1 (EEA1) is a marker of early endosomes and has an important role in endosomal trafficking. EEA1 contains coiled-coil regions and an FYVE-type zinc finger which interacts with phosphatidylinositol-3-phosphate. EEA1 functions as a Rab5 effector, mediates endosome docking and, together with SNAREs, leads to membrane fusion.

Protocole

Product Specific Protocols
WB protocol for EEA1 antibody 22266-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Immunity

The Endotoxin Delivery Protein HMGB1 Mediates Caspase-11-Dependent Lethality in Sepsis.

Authors - Meihong Deng
View Article
human

EMBO J

FUNDC1 regulates mitochondrial dynamics at the ER-mitochondrial contact site under hypoxic conditions.

Authors - Wenxian Wu
View Article

Int J Cancer

TGF-β induced PAR-1 expression promotes tumor progression and osteoclast differentiation in giant cell tumor of bone.

Authors - Ting Wang
View Article
human

J Exp Clin Cancer Res

Temozolomide-Perillyl alcohol conjugate impairs Mitophagy flux by inducing lysosomal dysfunction in non-small cell lung Cancer cells and sensitizes them to irradiation.

Authors - Minghui Chang
View Article
human

Virol J

A GFP-tagged version of the pseudorabies virus protein UL56 localizes to the Golgi and trans-Golgi network through a predicted C-terminal leucine-rich helix in transfected cells.

Authors - Chuang Lyu
View Article
human

Biochem Biophys Res Commun

Identification and characterization of an endoplasmic reticulum localization motif.

Authors - Yong-Wei Nie
View Article