Anticorps Polyclonal de lapin anti-PHD2/EGLN1

PHD2/EGLN1 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 20368-1-AP

Synonymes

C1orf12, ECYT3, Egl nine homolog 1, EGLN1, HIF PH2, HIF prolyl hydroxylase 2, HIFPH2, HPH 2, HPH2, PHD2, PHD2/EGLN1, PNAS 137, SM 20, SM20, ZMYND6

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Galerie de données de validation

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  WB analysis of HEK-293 using 20368-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20368-1-AP (PHD2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 20368-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 20368-1-AP (PHD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 20368-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20368-1-AP (PHD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 20368-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20368-1-AP (PHD2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas using 20368-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 20368-1-AP (PHD2 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human pancreas using 20368-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 20368-1-AP (PHD2 antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human pancreas using 20368-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 20368-1-AP (PHD2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human pancreas using 20368-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 20368-1-AP (PHD2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human pancreas using 20368-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 20368-1-AP (PHD2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 20368-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 20368-1-AP (PHD2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 20368-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 20368-1-AP (PHD2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 20368-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 20368-1-AP (PHD2 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 20368-1-AP

  Immunofluorescent analysis of HepG2 cells, using EGLN1 antibody 20368-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HepG2, tissu pancréatique de souris
• Résultats positifs en IHC: tissu pancréatique humain, tissu cardiaque humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

20368-1-AP cible PHD2/EGLN1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: egl nine homolog 1 (C. elegans)
• Masse moléculaire calculée: 46 kDa
• Poids moléculaire observé: 46 kDa, 36 kDa
• Numéro d’acquisition GenBank: NM_022051
• Symbole du gène: EGLN1
• Identification du gène (NCBI): 54583
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EGLN1, also named as PHD2, SM-20, HPH-2 and HIF-PH2, catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. It hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. EGLN1 functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Defects in EGLN1 are the cause of erythrocytosis familial type 3 (ECYT3). EGLN1 has 3 isoforms with MW of 46 kDa, 44 kDa and 36 kDa produced by alternative splicing. It mainly localizes in cytoplasm and can shuttle between the nucleus and cytoplasm (PubMed:19631610). The antibody is specific to EGLN1.

Publications

Species	Application	Title
human	WB	Oncogene; iASPP is essential for HIF-1α stabilization to promote angiogenesis and glycolysis via attenuating VHL-mediated protein degradation.; Authors - Dong Zhao
human	WB	Asian J Androl; CHD1 deletion stabilizes HIF1α to promote angiogenesis and glycolysis in prostate cancer; Authors - Yu-Zhao Wang